A rapid non-destructive DNA extraction method for insects and other arthropods

Castalanelli, Mark A.; Severtson, Dustin; Brumley, Cameron; Szito, A.; Foottit, Robert G.; Grimm, Mike; Munyard, Kylie; Groth, David

doi:10.1016/j.aspen.2010.04.003

Cited by 70 publications

(56 citation statements)

References 18 publications

(29 reference statements)

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“…Only one has used members from the Hemiptera and these were samples that were less than four years old [50]. However, mtCOI has been amplified from beetles from museum collections dating back to 1820.…”

Section: Discussionmentioning

confidence: 99%

Will the Real Bemisia tabaci Please Stand Up?

Tay

Evans²,

Boykin

et al. 2012

PLoS ONE

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Since Panayiotis Gennadius first identified the whitefly, Aleyrodes tabaci in 1889, there have been numerous revisions of the taxonomy of what has since become one of the world's most damaging insect pests. Most of the taxonomic revisions have been based on synonymising different species under the name Bemisia tabaci. It is now considered that there is sufficient biological, behavioural and molecular genetic data to support its being a cryptic species complex composed of at least 34 morphologically indistinguishable species. The first step in revising the taxonomy of this complex involves matching the A. tabaci collected in 1889 to one of the members of the species complex using molecular genetic data. To do this we extracted and then amplified a 496 bp fragment from the 3′ end of the mitochondrial DNA cytochrome oxidase one (mtCOI) gene belonging to a single whitefly taken from Gennadius' original 1889 collection. The sequence identity of this 123 year-old specimen enabled unambiguous assignment to a single haplotype known from 13 Mediterranean locations across Greece and Tunisia. This enabled us to unambiguously assign the Gennadius A. tabaci to the member of the B. tabaci cryptic species complex known as Mediterranean or as it is commonly, but erroneously referred to, as the ‘Q-biotype’. Mediterranean is therefore the real B. tabaci. This study demonstrates the importance of matching museum syntypes with known species to assist in the delimitation of cryptic species based on the organism's biology and molecular genetic data. This study is the first step towards the reclassification of B. tabaci which is central to an improved understanding how best to manage this globally important agricultural and horticultural insect pest complex.

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Section: Discussionmentioning

confidence: 99%

Will the Real Bemisia tabaci Please Stand Up?

Tay

Evans²,

Boykin

et al. 2012

PLoS ONE

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“…Whiteßy samples were stored at Ϫ20ЊC before DNA extraction. DNA was extracted from whole specimens with XytXtract Insect (ANDE; Xytogen; Perth, Australia) DNA extraction kits using manufacturer-recommended protocols (Castalanelli et al 2010). When possible, whiteßy specimens were retained and stored individually in 95% ethanol.…”

Section: Methodsmentioning

confidence: 99%

Demographic Trends in Mixed <I>Bemisia tabaci</I> (Hemiptera: Aleyrodidae) Cryptic Species Populations in Commercial Poinsettia Under Biological Control- and Insecticide-Based Management

et al. 2014

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Bemisia tabaci (Gennadius) is an economically important pest of agricultural and ornamental plants worldwide and is now widely recognized as a cryptic species complex. In North America, B. tabaci is a particularly important pest of greenhouse poinsettia. In poinsettia production, two cryptic species from the B. tabaci complex, Mediterranean and Middle East Minor 1, often infest crops simultaneously. Differences in pesticide susceptibility between these two cryptic species have the potential to influence growers' management decisions, including the use of biological control or insecticides, and the choice of insecticide active ingredient. However, the demographic behavior of mixed-species infestations in commercial greenhouses has yet to be investigated. We conducted a survey of B. tabaci populations in commercial greenhouses in Ontario, Canada, and provide evidence that under biological control-based management, Middle East Minor 1 can displace Mediterranean, whereas under insecticide-based management Mediterranean populations will persist. Furthermore, we comment on implications of this behavior on the management of B. tabaci, and comment on methods used to identify B. tabaci cryptic species.

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“…Rapid, non‐destructive DNA extraction techniques for arthropods (Castalanelli et al. ) allow for complete analysis from sample to identification in the field for insect pests, while preserving the sample as a voucher for further morphological analysis.…”

Section: Introductionmentioning

confidence: 99%

Real‐time PCR for identification of the soybean aphid, Aphis glycines Matsumura

Naaum

Foottit

Maw

et al. 2014

J Applied Entomology

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The soybean aphid (Aphis glycines Matsumura) is an economically significant pest in North America, causing extensive damage to soybean crops through direct feeding damage and disease transmission. If unchecked, this pest could cause billions of dollars of damage to soybean crops. Identification of the soybean aphid can be difficult due to its small size, complex life cycle and morphological plasticity. Generally, an expert is required to identify a specimen. Additionally, identification of some life stages, such as eggs, is impossible. DNA barcoding has been successfully used to differentiate aphid species, including A. glycines, based on sequencing of a standardized gene region. Although this method represents an important step towards accurate identification, samples must still be sent to specialized facilities for analysis. Using existing DNA barcode sequences in the publically accessible Barcode of Life Data System (BOLD; www.boldsystems. org), species-specific differences were identified and used to develop a realtime PCR assay to identify soybean aphids. This assay can be run on portable systems for rapid, accurate and simple identification in the field. The use of a non-destructive DNA extraction protocol allows the original insect to be vouchered and therefore available for further study if necessary. This work represents an important step in soybean aphid management.

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A rapid non-destructive DNA extraction method for insects and other arthropods

Cited by 70 publications

References 18 publications

Will the Real Bemisia tabaci Please Stand Up?

Will the Real Bemisia tabaci Please Stand Up?

Demographic Trends in Mixed <I>Bemisia tabaci</I> (Hemiptera: Aleyrodidae) Cryptic Species Populations in Commercial Poinsettia Under Biological Control- and Insecticide-Based Management

Real‐time PCR for identification of the soybean aphid, Aphis glycines Matsumura

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