2020
DOI: 10.1016/j.psj.2020.03.045
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A rapid novel visualized loop-mediated isothermal amplification method for Salmonella detection targeting at fimW gene

Abstract: Salmonella infection causes huge losses in the poultry industry worldwide. With the aim to prevent infectious diseases caused by Salmonella and to achieve rapid visualized Salmonella detection in poultry production, we used cresol red as an indicator to develop a novel visualized loop-mediated isothermal amplification method that targets the Salmonella fimW gene firstly in related field. The detection limit was 7.3 × 10 … Show more

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Cited by 22 publications
(9 citation statements)
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“…Several Salmonella LAMP assays, mostly targeting the invA gene, have been applied in various food and feed matrices, including chicken, turkey, pork, beef, and produce, as shown in a comprehensive review (50) and a few recent studies (18,47,48). Using spiked samples, one assay was able to directly detect (i.e., no enrichment) 10 2 CFU/mL Salmonella in raw beef, pork, chicken, lettuce, vegetable salad, watermelon, and other foods (39), whereas another assay reached the same level of detection in raw pork and milk with 24-h enrichment (19).…”
Section: Discussionmentioning
confidence: 99%
“…Several Salmonella LAMP assays, mostly targeting the invA gene, have been applied in various food and feed matrices, including chicken, turkey, pork, beef, and produce, as shown in a comprehensive review (50) and a few recent studies (18,47,48). Using spiked samples, one assay was able to directly detect (i.e., no enrichment) 10 2 CFU/mL Salmonella in raw beef, pork, chicken, lettuce, vegetable salad, watermelon, and other foods (39), whereas another assay reached the same level of detection in raw pork and milk with 24-h enrichment (19).…”
Section: Discussionmentioning
confidence: 99%
“…Another bacterial rapid test, the colloidal gold method, has simple operation and short reaction time, but it is prone to false positives or false negatives and has low sensitivity and specificity. To compensate for these shortcoming, research into the rapid detection of pathogenic bacteria using molecular biology techniques (such as PCR, gene chip, strand replacement technologies, autonomous replicating sequences, and others) has made great progress (12)(13)(14)(15)(16)(17)(18)(19); nonetheless, modern detection still requires expensive nucleic acid amplification equipment, highly trained personnel, complex procedures, and a detection time of 3-5 hours. Given these restrictions, it can only be performed in large medical institutions, and its application as a rapid diagnostic method is also limited.…”
Section: Discussionmentioning
confidence: 99%
“…Currently, the detection of pathogenic bacteria in clinical practice mainly relies on conventional culture methods, such as the examination of body fluids, blood, and secretions (5,6); the cultivation and identification of pathogenic bacteria; and drug sensitivity tests. In recent years, research into the rapid detection of pathogens using molecular biology methods has made great progress (7)(8)(9)(10). However, this detection requires expensive nucleic acid amplification equipment, with high requirements on laboratories and personnel, complicated procedures, and 3-5 hours of detection time.…”
Section: Discussionmentioning
confidence: 99%