A rapid preparative method for the purification of vitamin E acetate by kinetic reverse phase chromatography

Abstract

Alpha-tocopherol or vitamin E is an essential food ingredient. Due to high purity requirement in food applications, it is majorly produced in the acetate form through chemical routes. However, the isolation of food grade vitamin E acetate (VEA) is critical due to the co-generation of close characteristic impurities in the production processes. The work undertaken here provides a new kinetic chromatography method for production of food grade VEA. In this work, suitable adsorbent for chromatography separation was selected based on shallow-bed binding-elution characteristics. Loading conditions were identified by frontal adsorption behavior. Kinetic parameters and bed height for the purification was predicted by establishing the relationship between bed height, purity and the relative separation factor (RSF). The RSF of 2.8 and corresponding bed height is predicted from the established relationship for the separation of VEA from impurities. The bed height of 170 cm with isocratic mobile phase of 2% (wt/wt) water in methanol provides 98 wt.% purity of VEA with recovery of 90%. This work has highlighted methodology based on kinetic parameters for the separation of close characteristic impurities from target molecule. This work also provided the application of simple handy tools like RSF and dual wavelength detection for the rapid development of preparative separation process for VEA and other such valuable components from unknown impurities.

Preparative separation of nebivolol isomers by improved throughput reverse phase tandem two column chromatography

“…The elution profiles of impurities and nebivolols for the adsorbents under investigation were generated from the HPLC analysis of the cumulative fractions collected during washing and elution from corresponding adsorbent. Rs and RSF parameters were estimated by the formulas defined by Equations and , respectively 26–28 where t rd , t rl , and t rp are the retention time of d‐nebivolol, l‐nebivolol, and polar impurity, respectively.…”

“…Rs and RSF parameters were estimated by the formulas defined by Equations 1 and 2, respectively. [26][27][28] R s = 1:18…”