A Rapid Procedure for the Isolation of Catharanthine, Vindoline and Vinblastine

Bashir, M.; Hafeez, Maria; Perveen, N; Fatima, Javeria; An, Mistry

doi:10.1055/s-2007-970000

Cited by 19 publications

(3 citation statements)

References 3 publications

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“…The isolation of VLB is laborious and costly, mainly due to its low content in the plant [5] and a large number of alkaloids coexist [6]. Fortunately, an effective synthetic approach, which directly couples catharanthine (CTA) and vindoline (VLD) to provide VLB, has been established by Ishikawa et al [7].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of vinblastine and its monomeric precursors vindoline and catharanthine in Catharanthus roseus by capillary electrophoresis–mass spectrometry

Chen

Zhang

et al. 2011

J of Separation Science

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Catharanthus roseus is an important dicotyledonous medicinal plant that contains various anticancer components, such as vinblastine (VLB) and its monomeric precursors (vindoline and catharanthine). A capillary electrophoresis-mass spectrometry (CE-MS) approach for the simultaneous determination of three components was developed in this work. Baseline separation for three components was achieved by using a running buffer consisting of 20 mM ammonium acetate and 1.5% acetic acid in <20 min. Quantification of three components was assigned in positive-ion mode at a protonated molecular ion [M+H](+). The CE-MS method was validated for linearity, sensitivity, accuracy and precision, and then used to determine the content of the above components. The detection limits of VLB, catharanthine and vindoline are 0.8, 0.1 and 0.1 μg/mL, respectively. The precision was not more than 4.54% and the mean recovery of the analytes was 95.04-97.04%. The CE-MS method was successfully applied to determine VLB and its monomeric precursors in real sample C. roseus.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of vinblastine and its monomeric precursors vindoline and catharanthine in Catharanthus roseus by capillary electrophoresis–mass spectrometry

Chen

Zhang

et al. 2011

J of Separation Science

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“…At present, VLB is mainly obtained by extraction from natural plants, because chemical total synthesis, hemisynthesis and other methods are not practical for large-scale production [4,5]. However, the content of VLB in the plant is low (about 0.004%), and separation and analysis of VLB from natural plants is difficult to practise using conventional separating materials, such as silica gel and alumina [6,7]. Recently centrifugal partition chromatography and supercritical fluid extraction have been reported for VLB isolation [8,9].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of molecularly imprinted polymers for the application of selective clean-up vinblastine from Catharanthus roseus extract

Zhu

Huang

et al. 2010

Sci. China Chem.

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Molecularly imprinted polymers (MIPs) are synthetic tailor-made polymers with high selectivity towards a particular substance (template). An MIP using vinblastine (VLB) as the template molecule was synthesized and characterized. The presence of monomer-template complexes in a non-covalent way was confirmed by UV-vis spectrometry analysis. The polymerization was performed using methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linking agent, and toluene as the porogenic solvent by a thermo-polymerization method. The characterization of the obtained MIP was evaluated by scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) analysis. It was observed that the morphology of the MIP was more porous and rough, and the surface area had a significant increase compared with that of the non-imprinted polymer (NIP). This MIP was used as the sorbents of solid-phase extraction (SPE) to assess the selectivity of the MIP after optimization of the SPE protocol. VLB was specifically adsorbed on the MIP cartridge, while to vincristine (VCR), the chemical analog of VLB, almost no selective binding appeared. On the basis of the results, Catharanthus roseus extract was applied to the MIP cartridge for investigating its capability to extract VLB from the plant extract, and the capacity of the MIP cartridge was also evaluated. It was shown that the MIP could effectively enrich VLB from C. roseus extract and the recovery amounted to 93.8%. The solvents dissolving the samples had significant influence on the capacity of the MIP cartridge; it was 750 g/g in toluene, 625 g/g in chloroform, and 250 g/g in methanol. molecular imprinting polymer, vinblastine, selective extraction, Catharanthus roseus extract

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“…Vinblastine and vinacristine are traditionally obtained from C. roseus and are an antimicrotubule drug used to treat more than one type of cancer [5]. They are anti-neoplastic medicines and the monoindole alkaloids ajmalicine and serpentine are antihypertension drugs [6][7][8][9][10][11][12]. Vinblastine sulphate is used commercially for the treatment of neoplasma and is recommended for generalized Hodgkin's disease and resistant choiocarcenoma.…”

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confidence: 99%

Effect of Catharanthus mosaic virus (CatMV) on antineoplastic alkaloids from periwinkle (Catharanthus roseus L.) cultured in Mecca region and resistance induction by plant growth-promoting rhizobacteria

Al-Zahrany

Elbeshehy

Aldhebiani

et al. 2019

Biotechnology & Biotechnological Equipment

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Periwinkle (Catharanthus roseus L.) grown in the Mecca region contains more than 100 alkaloids such as vincristine and vinblastine used to treat leukaemia. The members of family Potyviridae, including Catharanthus mosaic virus (CatMV), cause natural infections in periwinkle. The presence of CatMV in collected samples was tested by double antibody sandwich enzyme linked immunosorbent assay and was confirmed by reverse transcription polymerase chain reaction using specific primers. The effect of CatMV infection on vincristine and vinblastine was determined by chromatographic techniques. All the isolated mixtures were evaluated by high-performance liquid chromatography. Vinblastine and vincristine were isolated by the use of a charcoal column. We evaluated the potential use of plant growth-promoting rhizobacterial isolates (Bacillus subtilis and Bacillus pumilus) against virus infection to stimulate resistance induction in host plants.

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A Rapid Procedure for the Isolation of Catharanthine, Vindoline and Vinblastine

Cited by 19 publications

References 3 publications

Simultaneous determination of vinblastine and its monomeric precursors vindoline and catharanthine in Catharanthus roseus by capillary electrophoresis–mass spectrometry

Simultaneous determination of vinblastine and its monomeric precursors vindoline and catharanthine in Catharanthus roseus by capillary electrophoresis–mass spectrometry

Synthesis of molecularly imprinted polymers for the application of selective clean-up vinblastine from Catharanthus roseus extract

Effect of Catharanthus mosaic virus (CatMV) on antineoplastic alkaloids from periwinkle (Catharanthus roseus L.) cultured in Mecca region and resistance induction by plant growth-promoting rhizobacteria

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