A rapid procedure to evaluate the dehydrogenase activity of soils low in organic matter

Klein, Donald A.; Loh, T.C.; Goulding, R. L.

doi:10.1016/0038-0717(71)90049-6

Cited by 274 publications

(107 citation statements)

References 3 publications

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“…Active microbial biomass carbon (AMBC) was measured by the glucose nutrient-induced respiration method (Islam and Weil, 2000). Soil dehydrogenase activity was determined using the method of Klein et al (1971). Phenol oxidase and peroxidase were measured with L-DOPA (L-3, 4 dihydroxyphenylalanine) as a substrate in an acetate buffer (Robertson et al, 1999).…”

Section: Soil Analysesmentioning

confidence: 99%

Assessment of environmental soil quality around Sonepur Bazari mine of Raniganj coalfield, India

et al. 2015

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Abstract. Assessment of soil quality is one of the key parameters for evaluation of environmental contamination in the mining ecosystem. To investigate the effect of coal mining on soil quality, opencast and underground mining sites were selected in the Raniganj coalfield area, India. The physical, chemical, and biological parameters of the soils, and trace metals and PAHs (polycyclic aromatic hydrocarbons) in the soils were evaluated. Soil dehydrogenase (+79 %) and fluorescein (+32 %) activities were significantly higher in underground mine (UGM) soil, whereas peroxidase activity (+57 %) was higher in opencast mine (OCM) soil. Content of As, Be, Co, Cr, Cu, Mn, Ni, and Pb was significantly higher in OCM soil, whereas Cd was higher in UGM. In general, the PAHs contents were higher in UGM soils, probably due to the natural coal burning at these sites. The observed values for the above properties were converted into a unitless score (0-1.00) and the scores were integrated into an environmental soil quality index (ESQI). In the unscreened index (ESQI-1) all the soil parameters were included and the results showed that the quality of the soil was better for UGM (0.539) than the OCM (0.511) soils. Principal component analysis was employed to derive ESQI-2 and accordingly, total PAHs, loss on ignition, bulk density, Be, Co, Cr, Ni, Pb, and microbial quotient (respiration: microbial biomass ratio) were found to be the most critical properties. The ESQI-2 was also higher for soils near UGM (+10.1 %). The observed indicators and the ESQI results revealed that soil quality assessment for these coal mining soils is largely depended on soil PAHs and potentially toxic trace metals. The proposed ESQI may be further refined by incorporating specific parameters related to human exposure risks and exposure pathways.

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Section: Soil Analysesmentioning

confidence: 99%

Assessment of environmental soil quality around Sonepur Bazari mine of Raniganj coalfield, India

et al. 2015

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“…The data was recorded on a number of soil biological properties such as soil microbial count, soil microbial biomass carbon, Dehydrogenase activity, CO 2 evolution and organic carbon. Soil microbial biomass carbon was determined by chloroform fumigation method as described by Jenkison and Powlson (1976), dehydroginase activity by Klein et al, (1971) and CO 2 evaluation of soil determined by alkali trap method of Anderson (1982). Organic carbon calculated by using method suggested by Walkely and Black (Nelson and Sommer, 1982).…”

Section: Methodsmentioning

confidence: 99%

Effect of Integrated Nutrient Management Practices on Soil Biological Properties in Acid Lime (Citrus aurentifolia Swingle) Orchard

Deshmukh¹,

Mishra²,

Bharad³

2018

Int.J.Curr.Microbiol.App.Sci

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“…Microbial biomass carbon was estimated in chloroform fumigation extraction procedure as described by Jenkinson and Powlson (1976) and calculation was made by Vance et al, (1987). Dehydrogenase activity in soil and vermicompost was determined by the spectrophotometer (Klein et al, 1971). Urease enzyme activity in samples was estimated by Tabatabai and Bremner (1972).…”

Section: Methodsmentioning

confidence: 99%

Comparative Assessment of Physicochemical and Biological Quality Characters of Vermicompost from Different Biomass Substrates

Kumar¹,

Paul²,

Kumar³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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A rapid procedure to evaluate the dehydrogenase activity of soils low in organic matter

Cited by 274 publications

References 3 publications

Assessment of environmental soil quality around Sonepur Bazari mine of Raniganj coalfield, India

Assessment of environmental soil quality around Sonepur Bazari mine of Raniganj coalfield, India

Effect of Integrated Nutrient Management Practices on Soil Biological Properties in Acid Lime (Citrus aurentifolia Swingle) Orchard

Comparative Assessment of Physicochemical and Biological Quality Characters of Vermicompost from Different Biomass Substrates

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