A rapid quantitative method of carisoprodol and meprobamate by liquid chromatography–tandem mass spectrometry

Essler, Shannon; Bruns, Kerry A.; Frontz, Michael; McCutcheon, J. Rod

doi:10.1016/j.jchromb.2012.09.001

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…Compared with ESI, we found that APCI afforded higher detection sensitivity for LC‐MS analyses of carisoprodol and meprobamate. APCI also is less prone to matrix effect for dilute samples than ESI .…”

Section: Resultsmentioning

confidence: 99%

Mass spectrometric analysis of carisoprodol and meprobamate in rat brain microdialysates

et al. 2016

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We report the evaluation of several mass spectrometry-based methods for the determination of carisoprodol and meprobamate in samples obtained from the rat brain by in vivo intracranial microdialyis. Among the techniques that aspire to perform analyses without chromatographic separation and thereby increase throughput, chip-based nanoelectrospray ionization and the use of an atmospheric pressure solids analysis probe fell short of requirements because of insufficient detection sensitivity and hard ionization, respectively. Although direct analysis in real time (DART) provided the required soft ionization, shortcomings of a tandem mass spectrometry-based assay also included inadequate detection sensitivity and, in addition, poor quantitative reproducibility. Therefore, liquid chromatography coupled with atmospheric-pressure chemical ionization tandem mass spectrometry was developed to determine carisoprodol and meprobamate from artificial cerebrospinal fluid as the medium. No desalting and/or extraction of the samples were necessary. The assay, combined with in vivo sampling via intracranial microdialyis, afforded time-resolved concentration profiles for the drug and its major metabolite from the nucleus accumbens region of the brain in rats after systemic administration of carisoprodol.

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Section: Resultsmentioning

confidence: 99%

Mass spectrometric analysis of carisoprodol and meprobamate in rat brain microdialysates

et al. 2016

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Quantitation of Carisoprodol and Meprobamate in Urine and Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Slawson

Johnson-Davis

2016

Methods in Molecular Biology

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Carisoprodol and meprobamate are centrally acting muscle relaxant/anxiolytic drugs that can exist in a parent-metabolite relationship (carisoprodol → meprobamate) or as a separate pharmaceutical preparation (meprobamate aka Equanil, others). The monitoring of the use of these drugs has both clinical and forensic applications in pain management applications and in overdose situations. LC-MS/MS is used to analyze urine or plasma/serum extracts with deuterated analogs of each analyte as internal standards to ensure accurate quantitation and control for any potential matrix effects. Positive ion electrospray is used to introduce the analytes into the mass spectrometer. Selected reaction monitoring of two product ions for each analyte allows for the calculation of ion ratios which ensures correct identification of each analyte, while a matrix-matched calibration curve is used for quantitation.

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Development of a novel seized drug screening method utilizing DART-MS and used weigh paper

Couch,

Tyler Davidson

2024

Forensic Chemistry

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A rapid quantitative method of carisoprodol and meprobamate by liquid chromatography–tandem mass spectrometry

Cited by 3 publications

References 15 publications

Mass spectrometric analysis of carisoprodol and meprobamate in rat brain microdialysates

Mass spectrometric analysis of carisoprodol and meprobamate in rat brain microdialysates

Quantitation of Carisoprodol and Meprobamate in Urine and Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Development of a novel seized drug screening method utilizing DART-MS and used weigh paper

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