1991
DOI: 10.1530/jrf.0.0910463
|View full text |Cite
|
Sign up to set email alerts
|

A rapid radioimmunoassay for determining plasma concentrations of LH in dogs

Abstract: Sepharose bead suspension containing anti-gamma-globulin, followed by incubation at room temperature for 30 min. The minimum detectable concentration in this assay, calculated from the precision profile, was 1\m=.\5 \ g=m\ g/ l . The amount of dog LH needed to cause 50% reduction of the initial binding was 1\m=.\57\ m=+-\0\m=.\13 ng/tube (15\m=.\7 \g=m\g/l for 100-\g=m\l samples). Daily blood samples were collected in heparinized tubes from the cephalic vein of 5 pointer and 7 beagle bitches from the onset of … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

1992
1992
2008
2008

Publication Types

Select...
2
1

Relationship

1
2

Authors

Journals

citations
Cited by 3 publications
(1 citation statement)
references
References 10 publications
0
1
0
Order By: Relevance
“…The plasma was removed and stored at -2OOC until assayed. Plasma luteinising hormone (LH) levels were measured using a heterologous dog LH radioimmunoassay (Smith and McDonald 1974, DePalatis and others 1978) modified by Madej and Linde-Forsberg (1991). The assay utilised radioiodinated ovine LH (LER-1056-C2), antiserum against ovine LH (GDN-15) at final dilution 1:150,000 and dog LH (LER-1685-1) as standard.…”
Section: Methodsmentioning
confidence: 99%
“…The plasma was removed and stored at -2OOC until assayed. Plasma luteinising hormone (LH) levels were measured using a heterologous dog LH radioimmunoassay (Smith and McDonald 1974, DePalatis and others 1978) modified by Madej and Linde-Forsberg (1991). The assay utilised radioiodinated ovine LH (LER-1056-C2), antiserum against ovine LH (GDN-15) at final dilution 1:150,000 and dog LH (LER-1685-1) as standard.…”
Section: Methodsmentioning
confidence: 99%