1989
DOI: 10.1271/bbb1961.53.1167
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A rapid screening method for alkaline .BETA.-cyclodextrin glucanotransferase using phenolphthalein-methyl orange-containing-solid medium.

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Cited by 38 publications
(23 citation statements)
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“…Screening of CGTase producing microorganisms was carried out by plate method described by Park et al with slight alteration. Horikoshi‐II agar plates were prepared by dissolving 10 g soluble starch, 5 g yeast extract, 5 g peptone, 1 g K 2 HPO 4 , and 0.2 g MgSO 4 .7H 2 O to 500 ml of distilled water .…”
Section: Methodsmentioning
confidence: 99%
“…Screening of CGTase producing microorganisms was carried out by plate method described by Park et al with slight alteration. Horikoshi‐II agar plates were prepared by dissolving 10 g soluble starch, 5 g yeast extract, 5 g peptone, 1 g K 2 HPO 4 , and 0.2 g MgSO 4 .7H 2 O to 500 ml of distilled water .…”
Section: Methodsmentioning
confidence: 99%
“…NPST-10 used in this study was recently isolated from hypersaline Soda Lakes, located in Wadi Natrun valley in northern Egypt (Ibrahim et al 2012). The bacterium was propagated in rich alkaline agar medium containing 0.02% (w/v) phenolphthalein, as an indicator of CGTase production (Park et al 1989). The alkaline agar medium (pH 10.5) contained soluble starch (10 g/l), yeast extract (5 g/l), casamino acids (5 g/l), peptone (5 g/l), NaCl (50 g/l), Na2CO3 (15 g/L), agar (15 g/l) and 300 μl trace elements solution.…”
Section: Methodsmentioning
confidence: 99%
“…SK13.002 originally was isolated from a soil sample in our laboratory and screened by phenolphthalein -methyl orange method, it was found to produce CGTase [9]. The crude enzyme was obtained after fermentation for 4 days and centrifugation at 10,000 rpm and 4 °C for 15 minutes.…”
Section: Partial Purification Of Cgtasementioning
confidence: 99%