2019
DOI: 10.1002/jssc.201900299
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A rapid, sensitive, and widely applicable method for quantitative analysis of underivatized amino acids in different biological matrices by UHPLC‐MS/MS

Abstract: A rapid, sensitive, and widely applicable method for the simultaneous quantitative analysis of 20 underivatized amino acids in different biological matrices, including serum, plasma, and tissue homogenates, using ultra high performance liquid chromatography with tandem mass spectrometry was developed and validated.Only 4 μL of serum, plasma, or tissue homogenate was extracted with 996 μL of solution (1.7 mM ammonium formate in 85% acetonitrile containing 0.1% formic acid) containing 100 ng/mL phenylalanine-d5 … Show more

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Cited by 17 publications
(9 citation statements)
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“…During the analytical process, quality control samples (QC) were regularly run to ensure result reproducibility, with peak area RSD for these QC samples being < 15% consistent with good stability and reproducibility. A total of 34 underivatized amino acids were assessed in patient and control serum samples [31]. Direct infusion mode was used for sample introduction into the mass spectrometer.…”
Section: Targeted Metabolomics Analysesmentioning
confidence: 99%
“…During the analytical process, quality control samples (QC) were regularly run to ensure result reproducibility, with peak area RSD for these QC samples being < 15% consistent with good stability and reproducibility. A total of 34 underivatized amino acids were assessed in patient and control serum samples [31]. Direct infusion mode was used for sample introduction into the mass spectrometer.…”
Section: Targeted Metabolomics Analysesmentioning
confidence: 99%
“…The precursor-to-product ion pair, cone voltage (CV), and collision energy (CE) for each analyte are given ( Supplementary Table S3 ). The details of targeted amino acid analyses are discussed in our previously published study ( Guo et al, 2019 ).…”
Section: Methodsmentioning
confidence: 99%
“…In positive ion mode [M + H] + , the precursor ion (Q1) is cracked by the energy to obtain the product ion (Q3) [24]. Cyt, Guad, Ino, Thyd, and Uri lose a ribose (132 Da) to get the corresponding product ion.…”
Section: Optimization Of Chromatographic Conditions and Ms Conditionsmentioning
confidence: 99%