A rapid serological assay for prediction of Salmonella infection status in slaughter pigs using surface plasmon resonance

Mazumdar, Saikat Datta; Barlen, Benjamin; Krämer, Thomas; Keusgen, Michael

doi:10.1016/j.mimet.2008.08.011

Cited by 20 publications

(9 citation statements)

References 25 publications

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“…Surface Plasmon resonance (SPR) technology is based on monitoring changes of refractive index on the sensor surface after ligand-biomolecule interaction. SPR biosensors have been used in detecting pathogenic microorganism causing microbial contamination, food spoilage and plant infection (Pellequer and Vanregenmortel, 1993;Deisingh and Thompson, 2004;Bergwerff and Van Knapen, 2006;Mazumdar et al, 2008;Dudak and Boyacı, 2009). The most important advantages of this technique rely on the label-free possibilities together with their ability to effectively measure/follow the bioaffinity reactions.…”

Section: Surface Plasmon Resonance (Spr) Systemsmentioning

confidence: 99%

Biosensors for plant pathogen detection

Khater

Escosura‐Muñiz

Merkoçi

2017

Biosensors and Bioelectronics

220

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Infectious plant diseases are caused by pathogenic microorganisms such as fungi, bacteria, viruses, viroids, phytoplasma and nematodes. Worldwide, plant pathogen infections are among main factors limiting crop productivity and increasing economic losses. Plant pathogen detection is important as first step to manage a plant disease in greenhouses, field conditions and at the country boarders. Current immunological techniques used to detect pathogens in plant include enzyme-linked immunosorbent assays (ELISA) and direct tissue blot immunoassays (DTBIA). DNA-based techniques such as polymerase chain reaction (PCR), real time PCR (RT-PCR) and dot blot hybridization have also been proposed for pathogen identification and detection. However these methodologies are time-consuming and require complex instruments, being not suitable for in-situ analysis. Consequently, there is strong interest for developing new biosensing systems for early detection of plant diseases with high sensitivity and specificity at the point-of-care. In this context, we revise here the recent advancement in the development of advantageous biosensing systems for plant pathogen detection based on both antibody and DNA receptors. The use of different nanomaterials such as nanochannels and metallic nanoparticles for the development of innovative and sensitive biosensing systems for the detection of pathogens (i.e. bacteria and viruses) at the point-of-care is also shown. Plastic and paper-based platforms have been used for this purpose, offering cheap and easy-to-use really integrated sensing systems for rapid on-site detection. Beside devices developed at research and development level a brief revision of commercially available kits is also included in this review.

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Section: Surface Plasmon Resonance (Spr) Systemsmentioning

confidence: 99%

Biosensors for plant pathogen detection

Khater

Escosura‐Muñiz

Merkoçi

2017

Biosensors and Bioelectronics

220

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“…Furthermore, assay tools using fluorescent dyes and quantum dots require expensive and complex signal readout instruments. Even though label-free assay methods have the advantage that they do not require labelling material and involve simple detection procedures, they have the weakness that they require advanced detection instruments to read out the assay results, such as those based on surface plasmon resonance, piezoelectricity, and electrochemical impedance spectroscopy 6,16,19 .…”

Section: Original Researchmentioning

confidence: 99%

Optical enzyme-linked immunosorbent assay on a strip for detection of Salmonella typhimurium

Park

Kim

2010

BioChip J

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A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) on an immunochromatographic strip was evaluated for the measurement of Salmonella typhimurium. Two different optical signal generation methods (colorimetric and chemiluminescent) were used to develop the proposed immunostrip system. Colorimetric and chemiluminescent signals could be successfully generated using the proposed immunostrip system, and S. typhimurium could be quantitatively measured in the range of 9.2×10 3 to 9.2×10 6 CFU/mL within 20 min (colorimetric method) and 16 min (chemiluminescent method). The selectivity and feasibility of the proposed immunostrip was confirmed by conducting experiments with spiked samples using real river water.

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“…Electrochemical Impedance Spectroscopy (EIS) [ 12 , 13 , 14 ], has been applied in detection of Salmonella [ 15 ] and E. coli [ 16 ]. Surface Plasmon Resonance (SPR) [ 17 , 18 , 19 , 20 ] and quartz crystal microbalances (QCM) [ 21 , 22 , 23 ] in Salmonella detection produced results in the range of 10 4 –10 5 colony forming units (cfu)/mL. Amperometric immunosensors have shown also the feasibility of detection of Salmonella and E. coli [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Protein Chips for Detection of Salmonella spp. from Enrichment Culture

Poltronieri

Cimaglia

Lorenzis

et al. 2016

Sensors

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Food pathogens are the cause of foodborne epidemics, therefore there is a need to detect the pathogens in food productions rapidly. A pre-enrichment culture followed by selective agar plating are standard detection methods. Molecular methods such as qPCR have provided a first rapid protocol for detection of pathogens within 24 h of enrichment culture. Biosensors also may provide a rapid tool to individuate a source of Salmonella contamination at early times of pre-enrichment culture. Forty mL of Salmonella spp. enrichment culture were processed by immunoseparation using the Pathatrix, as in AFNOR validated qPCR protocols. The Salmonella biosensor combined with immunoseparation showed a limit of detection of 100 bacteria/40 mL, with a 400 fold increase to previous results. qPCR analysis requires processing of bead-bound bacteria with lysis buffer and DNA clean up, with a limit of detection of 2 cfu/50 μL. Finally, a protein chip was developed and tested in screening and identification of 5 common pathogen species, Salmonella spp., E. coli, S. aureus, Campylobacter spp. and Listeria spp. The protein chip, with high specificity in species identification, is proposed to be integrated into a Lab-on-Chip system, for rapid and reproducible screening of Salmonella spp. and other pathogen species contaminating food productions.

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A rapid serological assay for prediction of Salmonella infection status in slaughter pigs using surface plasmon resonance

Cited by 20 publications

References 25 publications

Biosensors for plant pathogen detection

Biosensors for plant pathogen detection

Optical enzyme-linked immunosorbent assay on a strip for detection of Salmonella typhimurium

Protein Chips for Detection of Salmonella spp. from Enrichment Culture

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