A rapid, simple, and humane method for submandibular bleeding of mice using a lancet

Golde, William T.; Gollobin, Peter; Rodrı́guez, Luis L.

doi:10.1038/laban1005-39

Cited by 396 publications

(297 citation statements)

References 2 publications

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“…Mouse peripheral blood samples taken from the retro-orbital plexus or submandibular vein 56 were analysed by automatic cell counter (Nihon-Kohden, Tokyo). Peripheral blood smears were stained with Wright-Giemsa for morphological observation or new methylene blue for reticulocyte count.…”

Section: Methodsmentioning

confidence: 99%

A mouse model of adult-onset anaemia due to erythropoietin deficiency

et al. 2013

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Erythropoietin regulates erythropoiesis in a hypoxia-inducible manner. Here we generate inherited super-anaemic mice (ISAM) as a mouse model of adult-onset anaemia caused by erythropoietin deficiency. ISAM express erythropoietin in the liver but lack erythropoietin production in the kidney. Around weaning age, when the major erythropoietin-producing organ switches from the liver to the kidney, ISAM develop anaemia due to erythropoietin deficiency, which is curable by administration of recombinant erythropoietin. In ISAM severe chronic anaemia enhances transgenic green fluorescent protein and Cre expression driven by the complete erythropoietin-gene regulatory regions, which facilitates efficient labelling of renal erythropoietin-producing cells. We show that the majority of cortical and outer medullary fibroblasts have the innate potential to produce erythropoietin, and also reveal a new set of erythropoietin target genes. ISAM are a useful tool for the evaluation of erythropoiesis-stimulating agents and to trace the dynamics of erythropoietin-producing cells.

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Section: Methodsmentioning

confidence: 99%

A mouse model of adult-onset anaemia due to erythropoietin deficiency

et al. 2013

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“…Mice were anesthetized with Zoletil 50 (Virbac, Carros, France; 10 mg/kg, i.m. ), and blood was drawn using the facial vein technique (19). Blood was allowed to clot for 10 min, followed by centrifugation at 3,000 g for 15 min to obtain serum.…”

Section: Biochemical Assaysmentioning

confidence: 99%

Hot Water Extract of Leather Carp (Cyprinus carpio nudus) Improves Exercise Performance in Mice

Lee

Harwanto

Park

et al. 2015

JFN

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ABSTRACT:The hot water extract of leather carp (Cyprinus carpio nudus) has been used as a nourishing tonic soup and as an aid for recovery from physical fatigue. In this study, we investigated the effect of leather carp extract on exercise performance in mice. Swimming endurance and forelimb grip strength were assessed following oral administration of the extract (once per day for 7 days) at a dose of 0.5 mg/10 L/g body weight. After 7 days, mice given the leather carp extract had significantly greater swimming endurance [105±18 s (P＜0.05); 52% longer than day 0] and forelimb grip strength [1.18±0.05 Newton (P＜0.01); 17% greater than day 0]. The extract increased muscle mass, but had little effect on body weight. Following the swimming exercise, blood glucose, glutathione peroxidase, and superoxide dismutase levels in extract-fed mice were significantly higher (145%, 131%, and 106%, respectively) than in the saline control group. Blood levels of high-density lipoprotein cholesterol were also significantly increased (128%) in mice given the extract compared to the controls. These results suggest that leather carp extract can improve physical exercise performance and prevent oxidative stress caused by exhaustive workouts.

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“…This can be done using a lancet to stick the submandibular vein just before it opens to the jugular vein (Golde et al, 2005). The volume of blood collected should be less than 0.3 mL (Golde et al, 2005).…”

Section: Isolating T Cellsmentioning

confidence: 99%

The Use of Flow Cytometry to Monitor T cell Responses in Experimental Models of Graft-Versus-Host Disease

Anthony¹,

Hadley²

2012

Clinical Flow Cytometry - Emerging Applications

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A rapid, simple, and humane method for submandibular bleeding of mice using a lancet

Abstract: Methods for obtaining blood samples from mice tend to be difficult, inhumane, or both. The authors describe an inexpensive, disposable, single-use lancet for submandibular bleeding of mice that allows investigators to quickly draw 0.2-0.5 ml of blood without the use of anesthesia.

Cited by 396 publications

References 2 publications

A mouse model of adult-onset anaemia due to erythropoietin deficiency

A mouse model of adult-onset anaemia due to erythropoietin deficiency

Hot Water Extract of Leather Carp (Cyprinus carpio nudus) Improves Exercise Performance in Mice

The Use of Flow Cytometry to Monitor T cell Responses in Experimental Models of Graft-Versus-Host Disease

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