A rapid, simple, high-performance liquid chromatography method for the clinical measurement of beta-lactam antibiotics in serum and interstitial fluid

Abstract

Background: Enhanced methods of therapeutic drug monitoring are required to support the individualisation of antibiotic dosing based on pharmacokinetics (PK) parameters. PK studies can be hampered by limited total serum...

A low-volume LC/MS method for highly sensitive monitoring of phenoxymethylpenicillin, benzylpenicillin, and probenecid in human serum

“…The recovery was 99–104% for penicillin-V, 98–103% for penicillin-G, 98–105% for penicillin-G IS, 99–106% for probenecid, and 94–103% for probenecid IS in serum samples, indicating that the matrix effect did not significantly interfere with the calibration, as IS corrected for any matrix effect. 2,20…”

“…The recovery was 99-104% for penicillin-V, 98-103% for penicillin-G, 98-105% for penicillin-G IS, 99-106% for probenecid, and 94-103% for probenecid IS in serum samples, indicating that the matrix effect did not signicantly interfere with the calibration, as IS corrected for any matrix effect. 2,20 Accuracy and precision were tested in three different concentrations (Qc1, Qc2 and Qc3, Tables 2-4). Precision (RSD) within-intra-day and inter-day run ranged from 1.5 to 5.4% for penicillin-V, 1.2 to 4.5% for penicillin-G and 0.8 to 4.9% for probenecid.…”

“…These ndings align with data reported in other published research. 2,16 Application to clinical samples…”

“…Penicillins, a class of beta-lactam antibiotic with a narrow spectrum, are commonly prescribed for the treatment and prevention of infection caused by both Gram-positive and Gram-negative cocci. 1,2 It is crucial to ensure proper dosing of penicillin to maintain clinical effectiveness and minimize toxicity, especially in the treatment of common infections, in accordance with the WHO Access, Watch, and Reserve (AWaRe) criteria. 3,4 Increasing resistance to penicillin among prevalent organisms like streptococci underscores the necessity for innovative strategies to optimize the use of narrow-spectrum frontline antimicrobials.…”

“…Penicillins, a class of beta-lactam antibiotic with a narrow spectrum, are commonly prescribed for the treatment and prevention of infection caused by both Gram-positive and Gram-negative cocci. 1,2…”