A Rapid, Simple Method for the Determination of Reduced, Dehydro-, and Total Ascorbic Acid in Biological Material

Schaffert, Roscoe R.; Kingsley, George R.

doi:10.1016/s0021-9258(18)71093-4

Cited by 176 publications

(8 citation statements)

References 20 publications

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“…93) Resorcinol test (Jones, 1947); paper chromatography (Block et a!., 1958, pp. 178, 182) Paper chromatography as for glycerol ; colorimetry (Schaffert and Kingsley, 1955) Paper chromatography as for glycerol ; Folin-Wu method (Natelson,p. 205) Paper chromatography (Block et al,p.…”

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confidence: 99%

Freezing Resistance in Some Northern Fishes

Gordon¹,

Amdur²,

Scholander³

1962

The Biological Bulletin

105

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Scholander et al. (1957) described two groups of marine fishes from Hebron Fjord, Labrador, which spend long periods at subfreezing temperatures. One group (deep-water fishes) appears to survive by remaining perpetually in the supercooled state. The second group (shallow-water fishes) is exposed to subfreezing temperatures for about two-thirds of each year and survives these periods by combining supercooling with some increase in the osmotic concentration of their body fluids. The former group lives in water depths such that ice is absent even during the coldest winters. The latter group, however, lives in shallowerareas and is therefore likely to encounter ice. Natural selection has presumably operated on this second group so that those fishes have survived best which have added some antifreeze to their blood, thereby reducing the possibility of their fatally freezing as a result of an accidental collision with some ice. Several species of Norwegian boreal and arctic fishes apparently also respond to subfreezing temperatures in much the same way as the shallow water fishes from Labrador (Eliassen ct al., 1960). The conditions of life faced by the shallow-water fishes in Labrador in winter give rise to several questions, such as : how can these fishes tolerate any degree of supercooling at all, living as they do in close proximity to ice (supercooled nonarctic fishes freeze rapidly when touched by a piece of ice (Scholander ct al., 1957) ) ;and what is the nature of the antifreeze substance? It is not NaCl. The present paper describes new observations bearing on these subjects. MATERIALS AND METHODSIn March, 1959, the present authors made a return visit to the Hebron Fjord.

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confidence: 99%

Freezing Resistance in Some Northern Fishes

Gordon¹,

Amdur²,

Scholander³

1962

The Biological Bulletin

105

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“…The use of a complexing agent has been employed for the separation and identification of AA and its oxidation products from foodstuff, biological tissues and fluids, and pharmaceutical preparations. The samples were either homogenized or extracted in 10% TCA containing thiourea, which keeps AA in the reduced form, according to the method of Schaffert and Kingsley (1955). A portion of the extract was treated with Norit, which converted AA to DHAA.…”

Section: Resultsmentioning

confidence: 99%

“…Sample Pretreatment. AA and its biologically active oxidation product DHAA were isolated from foodstuffs (fresh orange and lime juices), tissues from guinea pigs fed high levels (2.5%) of AA (liver, kidney, and eye lens) (Roomi and Tsao, 1995), biological fluid from guinea pig (Roomi and Tsao, 1996) (urine and plasma), and pharmaceutical preparations of ascorbic acid (Bronson Pharmaceutical, St. Louis, MO) according to the method of Schaffert and Kingsley (1955). The samples were applied to the D-, B-, RP-, and RP-B silica and cellulose plates and developed as described above.…”

Section: Materials Andmentioning

confidence: 99%

Thin-Layer Chromatographic Separation of Isomers of Ascorbic Acid and Dehydroascorbic Acid as Sodium Borate Complexes on Silica Gel and Cellulose Plates

Roomi¹,

Tsao²

1998

J. Agric. Food Chem.

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A thin-layer chromatography (TLC) method is described for isomers of ascorbic acid (AA) and their oxidation product, dehydroascorbic acid (DHAA), on sodium borate impregnated (B) silica gel and cellulose plates. Borate complexes of AA and DHAA could be separated from each other, with AA migrating further. For comparative purposes, TLC was also attempted using direct (D), reversed-phase (RP), and reversed-phase sodium borate (RP-B) TLC plates with and without metaphosphoric acid (MPA). A reasonable separation of the three AA isomers is achieved on D and RP cellulose plates. Using an RP−silica−MPA plate the three AA isomers as well as the three DHAA isomers are fairly separated from their own group members. The separation of six stereoisomers, three AA isomers, and three DHAA is achieved using a D-cellulose−MPA plate. This procedure has been adopted to separate and identify AA and its oxidation product DHAA in food products, pharmaceutical preparations, and biological tissues and fluids. Keywords: Ascorbic acid; borate complexes; metaphosphoric acid

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“…The polyphenol and flavonoid contents of PK were analyzed using Folin-Ciocalteu and Vanillin sulfuric acid reagents, respectively (Thimmaiah, 1999). The total ascorbic acid content was estimated using 2, 4-dinitrophenyl hydrazine reagent (Schaffert and Kingsley, 1955). Total antioxidant power in terms of FRAP value was determined using TPTZ (2,4, 6-Tri (2 0 -pyridyl) 1,3,5-triazine) reagent (Benzie and Strain, 1996).…”

Section: Phytochemical Analysesmentioning

confidence: 99%

“…The lung lipid peroxidation (malondialdehyde concentration) was determined by the thiobarbituric acid (TBA) assay (Niehaus and Samuelsson, 1968). Total ascorbic acid was estimated using 2, 4-dinitrophenyl hydrazine reagent (Schaffert and Kingsley, 1955). Superoxide dismutase (SOD; EC 1.15.1.1) was measured using the nitroblue tetrazolium reduction method (Kakkar et al, 1984).…”

Section: Biochemical Analysesmentioning

confidence: 99%

Antioxidant and ACE enhancing potential of Pankajakasthuri in fluoride toxicity: Anin vitrostudy on mammalian lungs

2011

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Fluoride toxicity occurs due to high concentrations of fluoride in water sources or anthropogenic causes. The aim of the present study was to investigate the effects of an Ayurvedic drug--Pankajakasthuri (PK)--in relation to fluoride-induced toxicity in mammalian lungs. The results indicated that sodium fluoride increased lipid peroxidation and decreased enzymatic and non-enzymatic antioxidants in a concentration-dependent manner in lungs. The antioxidant potential of the lungs was suppressed maximally at 10 ppm fluoride concentration and PK at all three dose levels (i.e., 100, 200 and 300 µl) decreased fluoride induced lipid peroxidation (p < 0.05) and increased the levels of total ascorbic acid, superoxide dismutase, catalase, reduced glutathione, glutathione peroxidase and FRAP values significantly (p < 0.05) in a dose-dependent manner. When PK was examined for its effects on angiotensin-converting enzyme (ACE) activity, in fluoride-induced toxicity, the ACE activity was found to increase (p < 0.0001) in lung homogenates with all three doses. This study indicates that PK, an Ayurvedic drug, improves mammalian lung function by increasing antioxidant potential and ACE activity under the conditions of fluoride toxicity.

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A Rapid, Simple Method for the Determination of Reduced, Dehydro-, and Total Ascorbic Acid in Biological Material

Cited by 176 publications

References 20 publications

Freezing Resistance in Some Northern Fishes

Freezing Resistance in Some Northern Fishes

Thin-Layer Chromatographic Separation of Isomers of Ascorbic Acid and Dehydroascorbic Acid as Sodium Borate Complexes on Silica Gel and Cellulose Plates

Antioxidant and ACE enhancing potential of Pankajakasthuri in fluoride toxicity: Anin vitrostudy on mammalian lungs

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