A rapid spectrophotometric assay for prostaglandin synthetase: Application to the study of non-steroidal antiinflammatory agents

Takeguchi, C.; Sih, Charles J.

doi:10.1016/s0090-6980(72)80021-2

Cited by 124 publications

(16 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tryptophan (200 pm), glutathione (200 pM) and catechol (O-hydroxyphenol; 100 pM) are inactive and the effect of 2-hydroxy oestradiol-17 ß-acetate, a compound similar in all respects to 2-hydroxy oestradiol except in the derivative at the 17ß-position, is minimal (Kelly & Abel, 1980a Kelly unpublished observations). This is in accordance with the finding that such oxidations often accompany PG synthetase (Takeguchi & Sih, 1972) and we should therefore interpret apparent inactivity of low levels of adrenalin with caution.…”

Section: Male Reproductive Tractsupporting

confidence: 92%

Prostaglandin synthesis in the male and female reproductive tract

Kelly¹

1981

Reproduction

View full text Add to dashboard Cite

Section: Male Reproductive Tractsupporting

confidence: 92%

Prostaglandin synthesis in the male and female reproductive tract

Kelly¹

1981

Reproduction

View full text Add to dashboard Cite

“…In addition, these enzyme preparations impose many technical difficulties [8] and the results obtained in different laboratories vary considerably Table 2 Effects of different drugs on PGE 2 release from mouse peritoneal macrophages. [2,3,12]. Since former trials to introduce in vitro models into the screening of anti-inflammatory drugs had also failed quite regularly [6] we have pointed out [13] that the distinct compartmentalization aspirin-like drugs undergo in vivo may be crucial to their suitability as antiinflammatory drugs.…”

Section: Discussionmentioning

confidence: 99%

“…Correspondingly, inhibition of prostaglandin synthesis has been adopted as a screening method for antiinflammatory compounds [2][3][4][5]. Recently, several doubts to this approach have been expressed.…”

Section: Introductionmentioning

confidence: 99%

Prostaglandin release from macrophages: An assay system for anti-inflammatory drugs in vitro

Glatt¹,

Kälin²,

Wagner³

et al. 1977

Agents and Actions

View full text Add to dashboard Cite

It is the intention of this communication to present a simple and reliable in vitro system for the evaluation of anti-inflammatory drugs. The system described appears to combine the advantages of two established procedures, i.e. measurement of the inhibition of prostaglandin (PG) synthesis in vitro using microsomal enzyme preparations and the measurement of the inhibitory effects of drugs on PG synthesis in vivo avoiding major limitations. We propose to measure the effect of drugs on the PG release from mouse peritoneal macrophages in vitro. The influence of culture conditions, phagocytosis and drugs on the PG release from these cells is described. We found that antibody-coated erythrocytes are especially suitable triggers of PG release, and that the release can be inhibited by steroidal and non-steroidal anti-inflammatory drugs in concentrations resembling their effective doses in vivo.

show abstract

“…Despite good inhibition of arachidonate cyclo-oxygenase in vitro [12][13][14][15] and some moderate antiinflammatory effects in animal models [16][17][18][19][20][21][22][23], none of these antioxidants has produced potent anti-inflammatory activity by the oral route. Several years ago we began structural modification of H-transfer chain-breaking antioxidants such as BHT, BHA, and ethoxyquin.…”

Section: Figurementioning

confidence: 99%

2,6-Di-tert-butyl-4-(2′-thenoyl)phenol(R-830): A novel nonsteroidal anti-inflammatory agent with antioxidant properties

Moore¹,

Swingle²

1982

Agents and Actions

View full text Add to dashboard Cite

R-830, a di-tert-butylphenol, has been shown to be anti-inflammatory in a number of animal models. These include conventional systems such as carrageenan-induced edema and adjuvant arthritis of the rat and ultraviolet-induced erythema in the guinea pig in which the acidic nonsteroidal anti-inflammatory drugs (e.g., indomethacin) are effective. The anti-inflammatory activity of R-830 has also been demonstrated in other models (e.g., graft vs. host reaction and reversed passive cutaneous Arthus reaction in the rat, contact sensitivity in the mouse) in which the acidic nonsteroidal drugs are not effective. In vitro, R-830 inhibits guinea pig lung lipoxygenase and bovine seminal vesicle cyclo-oxygenase. The antioxidant properties of R-830 were demonstrated in two in vitro systems. We speculate that the antioxidant activity of this molecule might be related to its unusual profile of pharmacological activity.

show abstract

A rapid spectrophotometric assay for prostaglandin synthetase: Application to the study of non-steroidal antiinflammatory agents

Cited by 124 publications

References 20 publications

Prostaglandin synthesis in the male and female reproductive tract

Prostaglandin synthesis in the male and female reproductive tract

Prostaglandin release from macrophages: An assay system for anti-inflammatory drugs in vitro

2,6-Di-tert-butyl-4-(2′-thenoyl)phenol(R-830): A novel nonsteroidal anti-inflammatory agent with antioxidant properties

Contact Info

Product

Resources

About