A rapid T<sub>1</sub> mapping method for assessment of murine kidney viability using dynamic manganese‐enhanced magnetic resonance imaging

Jiang, Kai; Tang, Hui; Mishra, Prasanna K.; Macura, Slobodan; Lerman, Lilach O.

doi:10.1002/mrm.27025

Cited by 11 publications

(12 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This might have been masked by the increased fluid content because of tubular dilation, as indicated by the elevated T 1 (3). A similar increase in kidney water content has been reported in FA-induced nephropathy (21) as well as other kidney diseases, including renal artery stenosis (11,14) and ischemia-reperfusion injury (10,24). The increased fluid content in the kidney might also have hampered the ability of MTI in detecting mild renal fibrosis, especially in the medulla.…”

Section: Discussionsupporting

confidence: 79%

Multiparametric MRI detects longitudinal evolution of folic acid-induced nephropathy in mice

Jiang

Ponzo

Tang

et al. 2018

American Journal of Physiology-Renal Physiology

Self Cite

View full text Add to dashboard Cite

The rodent model of folic acid (FA)-induced acute kidney injury (AKI) provides a useful model for studying human AKI, but little is known about longitudinal changes in renal hemodynamics and evolution of renal fibrosis in vivo. In this work, we aimed to longitudinally assess renal structural and functional changes using multi-parametric magnetic resonance imaging (MRI). Ten adult mice were injected with FA, after which multi-parametric MRI was used to measure kidney volume, hypoxia index R, magnetization transfer ratio (MTR), perfusion, T, and glomerular filtration rate (GFR) at 2 weeks post-treatment. Then five mice were euthanized for histology and the other five underwent MRI again at 4 weeks followed by histology. Control mice (n=5) were injected with vehicle and studied with MRI at 2 weeks. Trichrome and hemotoxylin and eosin staining were performed to assess FA-induced tissue injuries. While kidney size and oxygenation showed progressive deterioration, a transient impairment in renal perfusion and normalized GFR slightly improved by 4 weeks. Kidney fluid content, as reflected by T, was prominent at 2 weeks and tended to regress at 4 weeks, consistent with observed tubular dilation. Trichrome staining revealed patchy necrosis and mild interstitial fibrosis at 2 weeks, which exacerbated at 4 weeks. MTR detected increased fibrosis at 4 weeks. In conclusion, multi-parametric MRI captured the longitudinal progression in kidney damage evolving within the first month after treatment with folic acid, and may provide a useful tool for assessment of therapeutic strategies.

show abstract

Section: Discussionsupporting

confidence: 79%

Multiparametric MRI detects longitudinal evolution of folic acid-induced nephropathy in mice

Jiang

Ponzo

Tang

et al. 2018

American Journal of Physiology-Renal Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…This study was approved by the Institutional Animal Care and Use Committee at our institution. Fifteen 10 week‐old male 129S1 mice underwent either sham ( n = 7) or RAS ( n = 8) surgeries on the right kidneys, as described previously 12,20–23 . Briefly, animals were maintained under anesthesia with 1.75% isoflurane mixed with O 2 .…”

Section: Methodsmentioning

confidence: 99%

“…A 10‐msec long adiabatic pulse (sech80) was used for nonselective inversion of magnetization. To ensure a high signal‐to‐noise ratio, a centric encoding scheme was implemented 22 . Other imaging parameters were: TR, 18 sec; TE, 4.9 msec; RARE factor, 16; recovery times, 0.023, 0.5, 1, 2, and 15 sec; number of averages, 1.…”

Section: Methodsmentioning

confidence: 99%

Quantitative Magnetization Transfer Detects Renal Fibrosis in Murine Kidneys With Renal Artery Stenosis

Jiang

Fang

Ferguson

et al. 2020

Magnetic Resonance Imaging

Self Cite

View full text Add to dashboard Cite

Background Renal fibrosis is a common pathway in tubulointerstitial injury and a major determinant of renal insufficiency. Collagen deposition, a key feature of renal fibrosis, may serve as an imaging biomarker to differentiate scarred from healthy kidneys. Purpose To test the feasibility of using quantitative magnetization transfer (qMT), which assesses tissue macromolecule content, to measure renal fibrosis. Study Type Prospective. Animal Model Fifteen 129S1 mice were studied 4 weeks after either sham (n = 7) or unilateral renal artery stenosis (RAS, n = 8) surgeries. Field Strength/Sequence Magnetization transfer (MT)‐weighted images were acquired at 16.4T using an MT‐prepared fast‐low‐angle‐shot sequence. Renal B0, B1, and T1 maps were also acquired, using a dual‐echo gradient echo, an actual flip angle, and inversion recovery method, respectively. Assessment A two‐pool model was used to estimate the bound water fraction (f) and other tissue imaging biomarkers. Masson's trichrome staining was subsequently performed ex vivo to evaluate renal fibrosis. Statistical Tests Comparisons of renal parameters between sham and RAS were performed using independent samples t‐tests. Pearson's correlation was conducted to investigate the relationship between renal fibrosis by histology and the qMT‐derived bound pool fraction f. Results The two‐pool model provided accurate fittings of measured MT signal. The qMT‐derived f of RAS kidneys was significantly increased compared to sham in all kidney zones (renal cortex [CO], 7.6 ± 2.4% vs. 4.6 ± 0.6%; outer medulla [OM], 8.2 ± 4.2% vs. 4.2 ± 0.9%; inner medulla [IM] + P, 5.8 ± 1.6% vs. 2.9 ± 0.6%, all P < 0.05). Measured f correlated well with histological fibrosis in all kidney zones (CO, Pearson's correlation coefficient r = 0.95; OM, r = 0.93; IM + P, r = 0.94, all P < 0.05). Data Conclusion The bound pool fraction f can be quantified using qMT at 16.4T in murine kidneys, increases significantly in fibrotic RAS kidneys, and correlates well with fibrosis by histology. Therefore, qMT may constitute a valuable tool for measuring renal fibrosis in RAS. Level of Evidence 1 Technical Efficacy Stage 3

show abstract

“…In ischemic mouse kidneys, this technique revealed decreased cellular viability. 58 This technique may afford noninvasive evaluation of renal viability, but it is limited by toxicity that prohibits clinical translation.…”

Section: Oxygenationmentioning

confidence: 99%

Basic principles and new advances in kidney imaging

Caroli

Remuzzi

Lerman

2021

Kidney International

Self Cite

View full text Add to dashboard Cite

A rapid T₁ mapping method for assessment of murine kidney viability using dynamic manganese‐enhanced magnetic resonance imaging

Cited by 11 publications

References 41 publications

Multiparametric MRI detects longitudinal evolution of folic acid-induced nephropathy in mice

Multiparametric MRI detects longitudinal evolution of folic acid-induced nephropathy in mice

Quantitative Magnetization Transfer Detects Renal Fibrosis in Murine Kidneys With Renal Artery Stenosis

Basic principles and new advances in kidney imaging

Contact Info

Product

Resources

About

A rapid T1 mapping method for assessment of murine kidney viability using dynamic manganese‐enhanced magnetic resonance imaging

Cited by 11 publications

References 41 publications

Multiparametric MRI detects longitudinal evolution of folic acid-induced nephropathy in mice

Multiparametric MRI detects longitudinal evolution of folic acid-induced nephropathy in mice

Quantitative Magnetization Transfer Detects Renal Fibrosis in Murine Kidneys With Renal Artery Stenosis

Basic principles and new advances in kidney imaging

Contact Info

Product

Resources

About

A rapid T₁ mapping method for assessment of murine kidney viability using dynamic manganese‐enhanced magnetic resonance imaging