A rapid tissue culture assay for the detection of okadaic acid and related compounds in mussels

Croci, Luciana; Cozzi, Loredana; Stacchini, A.; Medici, Dario De; Toti, L.

doi:10.1016/s0041-0101(96)00124-9

Cited by 25 publications

(13 citation statements)

References 10 publications

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“…Nonetheless, this method still lacks sufficient sensitivity to completely replace the MBA [14]. Similarly, alternative detection strategies based on molecular methodologies have been put forward, including cytotoxic assays based on the study of morphological changes of cultured cell lines exposed to OA [15,16,17]. Such approaches provide increased levels of sensitivity in the detection of OA while abolishing the use of test laboratory animals.…”

Section: Methods Used For the Detection Of Okadaic Acidmentioning

confidence: 99%

Okadaic Acid Meet and Greet: An Insight into Detection Methods, Response Strategies and Genotoxic Effects in Marine Invertebrates

et al. 2013

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Harmful Algal Blooms (HABs) constitute one of the most important sources of contamination in the oceans, producing high concentrations of potentially harmful biotoxins that are accumulated across the food chains. One such biotoxin, Okadaic Acid (OA), is produced by marine dinoflagellates and subsequently accumulated within the tissues of filtering marine organisms feeding on HABs, rapidly spreading to their predators in the food chain and eventually reaching human consumers causing Diarrhetic Shellfish Poisoning (DSP) syndrome. While numerous studies have thoroughly evaluated the effects of OA in mammals, the attention drawn to marine organisms in this regard has been scarce, even though they constitute primary targets for this biotoxin. With this in mind, the present work aimed to provide a timely and comprehensive insight into the current literature on the effect of OA in marine invertebrates, along with the strategies developed by these organisms to respond to its toxic effect together with the most important methods and techniques used for OA detection and evaluation.

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Section: Methods Used For the Detection Of Okadaic Acidmentioning

confidence: 99%

Okadaic Acid Meet and Greet: An Insight into Detection Methods, Response Strategies and Genotoxic Effects in Marine Invertebrates

et al. 2013

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“…A comparison of BGM and LLC-PK1 cells for the evaluation of nephrotoxicity toxicological studies, basically in studies of dysentery shellfish (Croci et al, 1997;Tan et al, 2011) and heavy metals (Romero et al, 2004). Experience in our laboratory has shown that the main advantage of using such cells is their ease of use.…”

Section: Research Articlementioning

confidence: 99%

A comparison of BGM and LLC-PK1 cells for the evaluation of nephrotoxicity

Tagliati¹,

Romero²,

Dutra³

et al. 2011

Drug and Chemical Toxicology

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Nephrotoxicity is one of the most frequent effects observed after the use of medicine. Such situations have been tardily discovered because of existing methods to determine toxicity. The validation of sensitive, alternative methods for the early identification of toxic effects is as important as restrictions on the use of animals. In this light, the present study evaluated the effects of gentamicin on BGM and LLC-PK1 cells, using MTT and Neutral Red (NR). Although the LLC-PK1 cell line is used for toxicological studies, the BGM cell line is relatively new for this purpose. MTT (BGM: EC(50) = 6.29 mM; LLC-PK1: EC(50) = 8.01 mM) was found to be more sensitive than NR (EC(50) was greater than 10 mM for both cells). By using MTT, both cells demonstrated the involvement of mitochondria in a manner that was dose dependent, with an apoptotic process occurring at the concentrations of 1 and 3 mM and necrosis at concentrations above 4 mM. It could, therefore, be concluded that 1) BGM appears to be useful in the study of the mechanism of nephrotoxicity caused by gentamicin and 2) because of its sensitivity to MTT, in addition to its ease of manipulation, it is believed that the BGM cell line can also be used as an alternative method to evaluate nephrotoxicity.

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“…A standard stock solution containing 20 μg OA ml –1 was prepared by dissolving the OA standard in methanol. An aliquot of the standard stock solution, suitably diluted, was used for the cytotoxicity test, as reported elsewhere (Croci et al . 1997).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of rapid methods for the determination of okadaic acid in mussels

Croci

Stacchini

Cozzi

et al. 2001

Journal of Applied Microbiology

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Two different screening methods, a Buffalo Green Monkey cytotoxicity test and a biosensor test, have been considered to replace the of®cial mouse bioassay in monitoring for okadaic acid (OA) levels in mussels. Methods and Results: Diarrhoetic shell®sh poison-contaminated mussels from the Adriatic Sea were assayed in parallel by means of the mouse bioassay and both alternative methods. Both the cytotoxicity test and the biosensor test showed high sensitivity (OA 0á01 mg g±1 hepatopancreas and 0á002 mg g±1 hepatopancreas, respectively) and a high correlation with the mouse bioassay (r 0á932, P < 0á001 and r ) 0á850, P < 0á001, respectively). Conclusions: Both methods are ef®cacious, quick, inexpensive and provide data on the amount of toxin present in mussels. Signi®cance and Impact of the Study: Both methods, besides allowing the simultaneous assay of a great number of samples, comply with the ethical need to reduce the use of animals in the laboratory

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A rapid tissue culture assay for the detection of okadaic acid and related compounds in mussels

Cited by 25 publications

References 10 publications

Okadaic Acid Meet and Greet: An Insight into Detection Methods, Response Strategies and Genotoxic Effects in Marine Invertebrates

Okadaic Acid Meet and Greet: An Insight into Detection Methods, Response Strategies and Genotoxic Effects in Marine Invertebrates

A comparison of BGM and LLC-PK1 cells for the evaluation of nephrotoxicity

Evaluation of rapid methods for the determination of okadaic acid in mussels

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