A rapid TLC autographic method for the detection of glucosidase inhibitors

Salazar, Mario O.; Furlán, Ricardo L. E.

doi:10.1002/pca.971

Cited by 58 publications

(39 citation statements)

References 18 publications

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“…A TLC method for the detection of ␤-glucosidase inhibitors which is based on the hydrolysis of esculin into esculetin (see sec- ferric chloride to provide a brown complex [57]. However, the method uses a natural product as enzymatic substrate, which may be difficult for the detection of inhibitors from plant extracts which contain other coumarin derivatives.…”

Section: 2˛-andˇ-glucosidase Inhibitionmentioning

confidence: 99%

Thin-layer chromatography with biological detection in phytochemistry

Marston

2011

Journal of Chromatography A

183

119

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Section: 2˛-andˇ-glucosidase Inhibitionmentioning

confidence: 99%

Thin-layer chromatography with biological detection in phytochemistry

Marston

2011

Journal of Chromatography A

183

119

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“…This method was also extrapolated to β-glucosidase using the respective enzyme and substrate, with a 20-min incubation at 37 ∘ C and a substrate/Fast Blue B salt ratio of 1 : 4. Another assay for β-glucosidase has been previously developed, based on the conversion of esculin into esculetin, which then reacts with FeCl 3 to provide a brown complex (Pandey et al, 2013;Salazar and Furlan, 2007). This assay was notably applied to chemically engineered plant extracts and led to the identification of a semisynthetic β-glucosidase inhibitor (Ramallo et al, 2012).…”

Section: -And -Glucosidase Inhibitionmentioning

confidence: 99%

Thin‐layer Chromatography of Plants, with Chemical and Biological Detection Methods

Urbain

Simões-Pires

2014

Encyclopedia of Analytical Chemistry

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Thin‐layer chromatography ( TLC ) is a widespread separation technique in the field of plant analysis and offers multiple advantages. This versatile technique can provide useful information about the qualitative or semiquantitative chemical composition of a mixture, thanks to coupling, with the detection and spectroscopic techniques and devices for image treatment. Moreover, it can contribute to the detection of biological activity by combining chromatography with bioassays, such as antimicrobial or enzyme inhibition tests. This procedure, providing simultaneous chromatographic separation and in situ assessment of a given biological activity, is named TLC bioautography. The major advantage of this technique is the ability to locate active substances from a complex mixture without prior sample purification. This avoids time‐consuming and tedious isolation of already‐known or inactive compounds. As this method is simple and cost effective, it is suitable for preliminary rapid screening to detect potential activity, as well as being a useful monitoring tool to track the bioguided isolation of active components. In this chapter, we focus on up‐to‐date TLC bioautographic assays, detail their mechanisms and applications in the field of natural drug discovery, and discuss the recent advances of this method that might appear at first glance to be a trivial approach.

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“…This method has been reported for screening of acetylcholinesterase [1,2,4,14,18], aldehyde dehydrogenase [13], cyclooxygenase-2 [7], glucosidase [19,24,25], lipase [10], monoamine oxidase [12] and xanthine oxidase [22], Phosphoglucose isomerase (D-glucose-6-phosphate aldose ketose-isomerase; PGI; EC 5.3.1.9) is an enzyme that plays an important role in the process of bacterial biofilm synthesis. PGI plays a crucial role in the biosynthesis of extracellular polysaccharides (EPS), the compound of microbial biofilm.…”

Section: Introductionmentioning

confidence: 99%

rapid detection for the inhibition of phosphoglucose isomerase from Escherichia coli by mercury(II) chloride based on TLC-autographic analysis – preliminary studies

Paradowska

Lutek

Ginalska

2014

Current Issues in Pharmacy and Medical Sciences

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The quest for new techniques for screening inhibitors of phosphoglucose isomerase is crucially important owing to therapeutic control of chronic bacterial infections associated with the biosynthesis of bacterial biofilm. According to the new method, yellowish zones against the purple background could be visually observed where phosphoglucose isomerase activity was inhibited. The new protocol with NADPH/NBT/PMS staining for TLC-autographic method was able to detect PGI inhibition by pure reference substance as mercury(II) chloride.

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A rapid TLC autographic method for the detection of glucosidase inhibitors

Abstract: A new bioautographic assay suitable for the localisation of beta-glucosidase inhibitors present in a complex matrix is described. Enzyme activity was detected using esculin as the substrate to produce esculetin, which reacts with ferric ion to form a brown complex.

Cited by 58 publications

References 18 publications

Thin-layer chromatography with biological detection in phytochemistry

Thin-layer chromatography with biological detection in phytochemistry

Thin‐layer Chromatography of Plants, with Chemical and Biological Detection Methods

rapid detection for the inhibition of phosphoglucose isomerase from Escherichia coli by mercury(II) chloride based on TLC-autographic analysis – preliminary studies

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