A Rapid, Validated RP-HPLC Method for the Determination of Seven Volatile N-Nitrosamines in Meat

Abstract

The present work reports a rapid reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of seven volatile N-nitrosamines namely N-nitrosodimethylamine (NDMA), N-nitrosomethylethylamine (NMEA), N-nitrosodiethylamine (NDEA), N-nitrosodiproylamine (NDPA), N-nitrosodi butylamine (NDBA), N-nitrosopiperidine (NPIP) and N-nitrosopyrrolidine (NPYR) for monitoring food safety. A strategic experimental approach was implemented for the method development. The desired chromatographic separation was achieved on a Symmetry C18 (4.6 × 150 mm, 5 µm) column using gradient elution. The optimized mobile phase consisted of the 10 mM ammonium hydroxide pH = 8.9 and acetonitrile. The eluted compounds were monitored at 231 nm wavelength using spectrophotometric detector. The developed method separated seven compounds from each other within a run time of 10 min. The method is effective for the determination of presence of these carcinogenic compounds. The average extraction recovery of seven nitrosamines was found 84.5%; the precision of method was found less than 2.7% and accuracy was found between 95% -102.5%. The assay could be applied in food monitoring safety.

Determination of Nitrate and Nitrite in Swiss Chard (Beta vulgaris L. subsp. vulgaris) and Wild Rocket (Diplotaxis tenuifolia (L.) DC.) and Food Safety Evaluations

“…Another important aspect of food safety related to nitrite intake is the possible formation of N-nitrosamines, a class of pro-carcinogenic compounds considered to be causally involved in the development of cancer in humans and animals, as first proposed by Barnes and Magee [ 11 ]. These compounds may also be found in food as the consequence of reaction between nitrosating agents with amino-based substances [ 12 , 13 ]. The most common nitrosating agents of food are nitrite salts and nitrogen oxides.…”

Quantification and Validation of a HPLC-UV Method for Simultaneous Analysis of Nitrosoamine Impurities (NDMA, NDEA and NDIPA) in Losartan

“…Moderate to high polarity stationary phases are used in these separations with carbowax or analogous material [17][18][19] . Alternatively, High Performance Liquid Chromatography (HPLC) on reverse phase columns have been mentioned for NDMA determination by Ultraviolet (UV) using diode array detection (DAD) at wavelength of 230 -233 nm [20][21][22] . Reproducible analyses of nitrosamines with pre column derivatization, ion chromatography and liquid chromatography using post column photolysis and chemiluminescence have also been utilized in past 23-27 23-27], therein giving rise to need of a new method which is simple, free of any complexities with respect to column, system and sample preparation and can be replicated easily for concurrent determination of all three nitrosamine impurities at low concentrations.…”

Determination of Genotoxic Impurities N-nitrosamine's in Efavirenz Drug Substance Using Rp-Hplc Technique

“…Moderate to high polarity stationary phases are used in these separations with carbowax or analogous material" [6][7][8]. "Alternatively, High Performance Liquid Chromatography (HPLC) on reverse phase columns have been mentioned for NDMA determination by Ultraviolet (UV) using diode array detection (DAD) at wavelength of 230-233 nm" [9][10][11].…”