A rare case of Ralstonia mannitolilytica infection in an end stage renal patient on maintenance dialysis during municipal water contamination

Lim, Christopher Thiam Seong; Lee, Sut Enn

doi:10.12669/pjms.334.13112

Cited by 24 publications

(27 citation statements)

References 8 publications

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“…In the present study, the genus Ralstonia (class Betaproteobacteria) was detected in both approaches and consisted of three species: R. insidiosa, R. solanacearum and Ralstonia sp. These findings were concordant with previous studies, which reported that these species were abundant and isolated from water, soil, and clinical samples (Ryan et al, 2006;Patton et al, 2009;Lim and Lee, 2017). Ralstonia possesses both chemoautotrophic and photoautotrophic properties and also plays a role in nitrogen fixation by oxidising ammonium to produce nitrite, making it available for various types of plants and benefitting the ecosystem (Lim and Lee, 2017).…”

Section: P R E S S Table 4 List Of Bacterial Species Identified Fromsupporting

confidence: 92%

A Comparative Study of Bacterial Communities Determined by Culture-Dependent and-Independent Approaches in Oil Palm Planted on Tropical Peatland

Ayob¹

2021

JOPR

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A combination of deoxyribonucleic acid (DNA)-based method and sequencing technologies have initiated a new era of soil microbial ecology to examine the patterns of bacterial communities in tropical peatland. The aims of the study are to verify and compare the bacterial communities in a 12-year-old oil palm plantation on peat of Sibu, Sarawak, Malaysia using culture-dependent and culture-independent approaches. The bacterial diversity identified from both approaches were amplified using 16S ribosomal deoxyribonucleic acid (rDNA) (341/907) primer, sequenced and analysed. This resulted in recovering a total of 227 bacterial isolates belonging to four major phyla accumulated from 22 genera. Meanwhile, about 216 denaturing gradient gel electrophoresis (DGGE) bands were excised, which corresponded to 195 different bacterial species from 20 different phyla by culture-independent method. Although both approaches detected a total of four predominant bacterial phyla (Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes), in general, different taxonomic sequences were targeted by each method. In comparison to culture-dependent, polymerase chain reaction-(PCR) DGGE method identified a higher rate of bacterial diversity and richness and also detected non-culturable bacteria. Thus, this suggests that culture-independent method was showed to be more efficient on the bacterial diversity identification that will lead towards unravelling the hidden bacterial species associated with agricultural practices carried out in Southeast Asia peatland.

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Section: P R E S S Table 4 List Of Bacterial Species Identified Fromsupporting

confidence: 92%

A Comparative Study of Bacterial Communities Determined by Culture-Dependent and-Independent Approaches in Oil Palm Planted on Tropical Peatland

Ayob¹

2021

JOPR

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“…3C and 4C). Surprisingly, compared to elders with AD, elders with other dementia types had higher proportions of some opportunistic pathogens, including Ralstonia pickettii and Ralstonia mannitolilytica (33, 34), as well as B. fragilis, the most commonly isolated anaerobic pathogen (35).…”

Section: Resultsmentioning

confidence: 98%

Alzheimer’s Disease Microbiome Is Associated with Dysregulation of the Anti-Inflammatory P-Glycoprotein Pathway

Haran

Bhattarai

Foley

et al. 2019

mBio

311

247

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The microbiota-gut-brain axis is a bidirectional communication system that is poorly understood. Alzheimer’s disease (AD), the most common cause of dementia, has long been associated with bacterial infections and inflammation-causing immunosenescence. Recent studies examining the intestinal microbiota of AD patients revealed that their microbiome differs from that of subjects without dementia. In this work, we prospectively enrolled 108 nursing home elders and followed each for up to 5 months, collecting longitudinal stool samples from which we performed metagenomic sequencing and in vitro T84 intestinal epithelial cell functional assays for P-glycoprotein (P-gp) expression, a critical mediator of intestinal homeostasis. Our analysis identified clinical parameters as well as numerous microbial taxa and functional genes that act as predictors of AD dementia in comparison to elders without dementia or with other dementia types. We further demonstrate that stool samples from elders with AD can induce lower P-gp expression levels in vitro those samples from elders without dementia or with other dementia types. We also paired functional studies with machine learning approaches to identify bacterial species differentiating the microbiome of AD elders from that of elders without dementia, which in turn are accurate predictors of the loss of dysregulation of the P-gp pathway. We observed that the microbiome of AD elders shows a lower proportion and prevalence of bacteria with the potential to synthesize butyrate, as well as higher abundances of taxa that are known to cause proinflammatory states. Therefore, a potential nexus between the intestinal microbiome and AD is the modulation of intestinal homeostasis by increases in inflammatory, and decreases in anti-inflammatory, microbial metabolism. IMPORTANCE Studies of the intestinal microbiome and AD have demonstrated associations with microbiome composition at the genus level among matched cohorts. We move this body of literature forward by more deeply investigating microbiome composition via metagenomics and by comparing AD patients against those without dementia and with other dementia types. We also exploit machine learning approaches that combine both metagenomic and clinical data. Finally, our functional studies using stool samples from elders demonstrate how the c microbiome of AD elders can affect intestinal health via dysregulation of the P-glycoprotein pathway. P-glycoprotein dysregulation contributes directly to inflammatory disorders of the intestine. Since AD has been long thought to be linked to chronic bacterial infections as a possible etiology, our findings therefore fill a gap in knowledge in the field of AD research by identifying a nexus between the microbiome, loss of intestinal homeostasis, and inflammation that may underlie this neurodegenerative disorder.

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“…bReferences for R . mannitolilytica 3–5,17,21,26–30. cOne strain was resistant to piperacillin and susceptible to piperacillin/tazobactam.…”

Section: Discussionmentioning

confidence: 99%

A case of persistent bacteraemia by Ralstonia mannitolilytica and Ralstonia pickettii in an intensive care unit

Basso¹,

Venditti²,

Raponi³

et al. 2019

IDR

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The Ralstonia spp . genus is a group of non-fermentative, Gram-negative bacteria often resistant to many antibiotics, which are emerging as opportunistic pathogens frequently associated with infections in hospital settings. We present herein a case of combined R. pickettii and R. mannitolilytica persisting and relapsing bacteraemia, possibly caused by a septic arterial thrombosis secondary to the rupture of an internal carotid artery aneurysm. Microbiology studies showed that both Ralstonia isolates produced biofilm and carried class D oxacillinase genes. When confronted with infections caused by members of the Ralstonia genus, identification to the species level is crucial for correct clinical management, as the two species show different antibiotic susceptibility patterns.

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A rare case of Ralstonia mannitolilytica infection in an end stage renal patient on maintenance dialysis during municipal water contamination

Cited by 24 publications

References 8 publications

A Comparative Study of Bacterial Communities Determined by Culture-Dependent and-Independent Approaches in Oil Palm Planted on Tropical Peatland

A Comparative Study of Bacterial Communities Determined by Culture-Dependent and-Independent Approaches in Oil Palm Planted on Tropical Peatland

Alzheimer’s Disease Microbiome Is Associated with Dysregulation of the Anti-Inflammatory P-Glycoprotein Pathway

<p>A case of persistent bacteraemia by <em>Ralstonia mannitolilytica</em> and <em>Ralstonia pickettii</em> in an intensive care unit</p>

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