A rare cause of sepsis: Lactococcus garvieae

Sahu, Kamal Kant; Sherif, Akil Adrian; Syed, Masood Pasha; Rajendran, Aruna; Mishra, Ajay Kumar; Davaro, Raul

doi:10.1093/qjmed/hcz078

Cited by 10 publications

(7 citation statements)

References 7 publications

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“…This bacterium was detected (positive rate: 40%, Table S2) and cultured (100%, Fig. 3 ) in our bat fecal samples ( 58 , 59 ), suggesting that we should increase discussion of Lactococcus spp. as potential pathogens, especially when they are detected in and cultured from wild animals, such as bats.…”

Section: Discussionmentioning

confidence: 88%

The Threat of Potentially Pathogenic Bacteria in the Feces of Bats

et al. 2022

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Section: Discussionmentioning

confidence: 88%

The Threat of Potentially Pathogenic Bacteria in the Feces of Bats

et al. 2022

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“…In view of this and other reports that described sepsis associated with L. garvieae infection [ 11 , 13 , 50 , 51 ], the knowledge of virulence factors and resistance mechanisms associated with the Lactococcus genera and L. garvieae species is detrimental.…”

Section: Discussionmentioning

confidence: 90%

Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate

Colagrossi

Costabile

Scutari

et al. 2022

Emerging Microbes & Infections

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Owing to an increasing number of infections in adults, Lactococcus (L.) garvieae has gained recognition as an emerging human pathogen, causing bacteraemia and septicaemia. In September 2020, four paediatric onco-hematologic patients received a platelet concentrate from the same adult donor at Bambino Gesù Children’s Hospital IRCCS, Rome. Three of four patients experienced L. garvieae sepsis one day after transfusion. The L. garvieae pediatric isolates and the donor’s platelet concentrates were retrospectively collected for whole-genome sequencing and shot-gun metagenomics, respectively (Illumina HiSeq). By de novo assembly of the L. garvieae genomes, we found that all three pediatric isolates shared a 99.9% identity and were characterized by 440 common SNPs. Plasmid pUC11C (conferring virulence properties) and the temperate prophage Plg-Tb25 were detected in all three strains. Core SNP genome-based maximum likelihood and Bayesian trees confirmed their phylogenetic common origin and revealed their relationship with L. garvieae strains affecting cows and humans (bootstrap values >100 and posterior probabilities = 1.00). Bacterial reads obtained by the donor’s platelet concentrate have been profiled with MetaPhlAn2 (v.2.7.5); among these, 29.9% belonged to Firmicutes, and 5.16% to Streptococcaceae (>97% identity with L. garvieae ), confirming the presence of L. garvieae in the platelet concentrate transfusion. These data showed three episodes of sepsis for the first time due to a transfusion-associated transmission of L. garvieae in three pediatric hospitalized hematology patients. This highlights the importance to implement the screening of platelet components with new human-defined pathogens for ensuring the safety of blood supply, and more broadly, for the surveillance of emerging pathogens.

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“…L. garvieae was firstly isolated from cows with mastitis and later characterized phenotypically and genetically as L. garvieae (Teixeira, 1996). "Lactococcus" genus has substantial importance to dairy and farm industry; it was reported that L. garvieae was isolated from goat cheese, raw cow milk, poultry, pork meat, and beef (Gibello, 2016;Sahu, 2019). In the 1950s, L. garvieae was determined on rainbow trout (Oncorhynchus mykiss) farms in Japan as well (Hoshina, 1958).…”

Section: Introductionmentioning

confidence: 99%

Lactococcus garvieae'nin Genomik Yaklaşımlarla Çeşitliliği

Kumru

2021

Journal of Anatolian Environmental and Animal Sciences

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Lactococcus garvieae is the Gram-positive cocci bacteria known as the causative agent of infectious systemic disease. It causes fatal hemorrhagic septicemia in mainly cultured fish species, animals, and humans worldwide. Comparative genome analyses provide valuable information about genome identification and unique genomic features. In this study, the available L. garvieae genomes are evaluated by comparative genomics approaches. The results indicated that there are four distinct genetic groups of L. garvieae based on the Average Nucleotide Identity (ANI) value. The phylogenetic tree that was produced using the 16S rRNA sequence has similar genetic variances with the publicly available L. garvieae genome data on NCBI and supports the ANI value. In silico analysis of antimicrobial resistance revealed that each L. garvieae genome groups have unique antimicrobial resistance class genes, even though, all genomes have a common antibiotic-resistant class. Analyzing results of the antimicrobial resistance supports the L. garvieae genetic variations. This extensively comparative approach will provide new insights into the understanding of L. garvieae genomic diversity and antimicrobial resistance

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A rare cause of sepsis: Lactococcus garvieae

Cited by 10 publications

References 7 publications

The Threat of Potentially Pathogenic Bacteria in the Feces of Bats

The Threat of Potentially Pathogenic Bacteria in the Feces of Bats

Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate

Lactococcus garvieae'nin Genomik Yaklaşımlarla Çeşitliliği

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