A rast assay for monitoring specific IgE antibodies in the mouse

Recent Insight into Class-Specific Properties of Murine Immunoglobulins Obtained with the Help of Monoclonal Antibodies

“…When this antiallotype Igh-7a was neutralized with great excess of antiovalbumin IgE, it still con tained a component neutralizing the immunizing IgE (clone B-52 or B-53) and this component was taken as [19,20], Murine IgE is one of the classes which can attach to mast cell receptors of the homologous species [9] and IgE can also attach to the receptors of rat mast cells [9,14], This property was therefore exploited to demonstrate and titrate murine IgE. With the advent of monoclonal antibodies; radio immunoassays [11,21,22] and enzyme-linked immu noassays [23] could be developed for detection of mu rine IgE [24], The main problem is the purity and the specificity of the anti-IgE antibody since trace amounts of antibody reacting with other immuno globulin classes or with the Fab fragment can be very troublesome. For this reason, an ELISA technique which uses monoclonal antiallotype antibody pre sents a tremendous advantage, [unpublished results from our and Dr. Tada's laboratory).…”