A ratiometric fluorescence method based on nitrogen-doped carbon quantum dots for the determination of the activity of alkaline phosphatase

A microfluidic approach to study variations in Chlamydomonas reinhardtii alkaline phosphatase activity in response to phosphate availability

“…28 Recently, Lv et al developed a ratiometric fluorescent approach using nitrogen-doped carbon quantum dots in a purified enzyme assay providing an alternative means to quantifying APA. 29 A significant limitation of this bulk-view approach lies in their tendency to quantify APA across an entire population, thereby reporting an average value that overlooks individual cell behavior. However, delving into single cell responsiveness offers a unique opportunity to uncover subpopulations with distinct behaviors, providing deeper insights into cellular dynamics and potential regulatory mechanisms.…”

A Fluorescence Biosensor for Tyrosinase Activity Analysis Based on Silicon-Doped Carbon Quantum Dots

“…The unique properties of CQDs, including simple preparation, excellent water solubility, and ease of surface functionalization, stable and tunable luminescence properties, make them highly promising candidates for fluorescence sensing [21][22][23] . CQDs have been successfully used to detect various biomolecules including trypsin [24] , hyaluronidase [25] , and alkaline phosphatase [26,27] . While, most of the fluorescent CQDs used for TYR activity detection still encounter the lack of low fluorescence quantum yield (QY) and poor sensitivity, which hinder the prospects of biological applications [28][29][30] .…”