A Ratiometric Fluorescent Probe for Hypochlorite Based on a Deoximation Reaction

Lin, Weiying; Long, Lingliang; Chen, Bingbing; Tan, Wen

doi:10.1002/chem.200802054

Cited by 248 publications

(106 citation statements)

References 33 publications

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“…Sodium hypochlorite (NaClO) is used as household bleach, for disinfection of drinking water and cooling-water treatment at a concentration in the range of 10 −5 to 10 −2 M [1]. As one of the important reactive oxygen species, hypochlorous acid (HClO), the protonated form of ClO − , functions as an antimicrobial agent for water treatment.…”

Section: Introductionmentioning

confidence: 99%

Copper nanocluster-based fluorescent probe for hypochlorite

2015

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Copper nanoclusters (Cu-NCs) were fabricated by chemical reduction of Cu(II) ions using formaldehyde as the reductant and poly(vinyl pyrrolidone) as the protecting agent. The resulting Cu-NCs were characterized by TEM, FT-IR, UV-vis and XPS and fluorescence spectroscopy. The CuNCs display a luminescence quantum yield of about 13 %, and the emission peaks shift from 398 to 457 nm on increasing the excitation wavelength from 310 to 390 nm. The Cu-NCs possess a storage stability of at least 2 months and are stable in the presence of high concentrations of salt. Their fluorescence is strongly quenched by hypochlorite, while other common cations, anions and hydrogen peroxide have minor (or no) effects on fluorescence. On this basis, a fluorometric hypochlorite assay was developed that has a 0.1 μM detection limit and a linear range that extends from 1 to 30 μM. The method was successfully used to the determination of hypochlorite in local tap water samples, and the results agreed well with those obtained by a colorimetric method.

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Section: Introductionmentioning

confidence: 99%

Copper nanocluster-based fluorescent probe for hypochlorite

2015

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“…or H 2 O 2 [8] or UV excitation [9] . Therefore, it has profound meanings to develop one bioimaging probe for HOCl with an excellent selectivity and sensitivity that is applicable to various biological systems.…”

Section: +mentioning

confidence: 99%

“…In addition, in conjunction with microscopy, bio-imaging applications can be used in living cells. Based on the reported ClO -probes [5][6][7][8][9] , they have the drawbacks like unsuitable pH range for cells [7,9] , lack of selectivity between ClO -and Cu…”

Section: Introductionmentioning

confidence: 99%

A specific fluorescence probe for hypochlorous acid based on rhodamin101 thiosemicarbazides and its application for living cells imaging

Zhu¹,

Han²,

Wu³

et al. 2016

Proceedings of the 2016 International Conference on Civil, Transportation and Environment

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Abstract. Utilizing the mechanism of HOCl-mediated cyclization of rhodamine 101 thiosemicarbazide (non-fluorescent) to rhodamine 101 oxadiazoles (fluorescent), we successfully developed a novel off-on far-red fluorescent probe ( 1 ) toward HOCl with high selectivity, great functionality at physiological pH and good cell-membrane permeability. Probe 1 was linearly proportional to the concentration of HOCl in the range of 1-10 M and the detection limit is 0.3 M. Moreover, the proposed probe (1) was successfully used for the detection of hypochlorous acid in HepG2 cells by fluorescent imaging.

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“…However, excess HOCl may cause tissue damage, and abnormal levels of HOCl are related to many pathological processes including cardiovascular disease, arthritis, neurodegeneration, and cancer [8][9][10][11]. To increase understanding of the biological effects of HOCl, many efforts have been made to develop selective and sensitive analytical methods for HOCl [1][2][3][12][13][14][15][16][17][18][19][20]. Among these methods, fluorescent probes have received considerable attention because of their good time and spatial resolution [21].…”

mentioning

confidence: 99%

“…Among these methods, fluorescent probes have received considerable attention because of their good time and spatial resolution [21]. To date, several fluorescent probes for HOCl sensing have been reported, which are designed based on HOClinduced oxidation reactions [12][13][14][15][16][17][18]. These probes exhibit reasonable sensitivity and selectivity.…”

mentioning

confidence: 99%

A water-soluble fluorescence resonance energy transfer probe for hypochlorous acid and its application to cell imaging

Jia

2011

Chin. Sci. Bull.

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A water-soluble fluorescence resonance energy transfer (FRET) probe for hypochlorous acid (HOCl), dansyl rhodamine B piperazinoacetohydrazide, was designed, synthesized and characterized. The dansyl moiety in the probe acted as a FRET donor and the rhodamine moiety acted as a FRET acceptor. The two moieties were connected by a HOCl-cleavable active bond, and cleavage of this linker decreased the FRET efficiency and increased the fluorescence intensity of the donor at 501 nm. The water solubility of the probe was improved compared with other probes by introduction of the cationic rhodamine fluorophore. As a result, the probe could be used to detect HOCl in aqueous biosystems with a linear range of 2-10 mol/L and a detection limit of 80 nmol/L (signal-to-noise = 3). The probe was successfully applied to fluorescence imaging of HOCl in HeLa cells.

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A Ratiometric Fluorescent Probe for Hypochlorite Based on a Deoximation Reaction

Cited by 248 publications

References 33 publications

Copper nanocluster-based fluorescent probe for hypochlorite

Copper nanocluster-based fluorescent probe for hypochlorite

A specific fluorescence probe for hypochlorous acid based on rhodamin101 thiosemicarbazides and its application for living cells imaging

A water-soluble fluorescence resonance energy transfer probe for hypochlorous acid and its application to cell imaging

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