A re-evaluation of the taxonomy of the genus Anaerovibrio, with the reclassification of Anaerovibrio glycerini as Anaerosinus glycerini gen. nov., comb. nov., and Anaerovibrio burkinabensis as Anaeroarcus burkinensis [corrig.] gen. nov., comb. nov.

Reclassification of Clostridium quercicolum as Dendrosporobacter quercicolus gen. nov., comb. nov.

“…described by Stro$ mpl et al (1999), and is consistent with their presence in other species in the SporomusaPectinatus-Selenomonas phyletic group (Helander et al, 1994 ;Kamio et al, 1972a, b). The overall fatty acid pattern was consistent with the pattern found within members of the Sporomusa-Pectinatus-Selenomonas phyletic group (Stro$ mpl et al, 1999), but also allows differentiation of C. quercicolum from other members of this evolutionary group (Miyagawa et al, 1979 ;Moore et al, 1994 ;Shah et al, 1983).…”

“…In contrast, significant levels of 3-hydroxy fatty acids were present (Table 1). Studies of members of the genus Anaerovibrio (Stro$ mpl et al, 1999) have shown that 3-hydroxy fatty acids may have been wrongly identified as dimethyl acetals in the work of Moore et al (1994). The identity of the 3-hydroxy fatty acids was confirmed by GC-MS, as ?, Unknown structure ; -, not detected.…”

“…2) comprised two major phospholipids and one major glycolipid. The two phospholipids were identified as phosphatidyl ethanolamine and phosphatidyl serine, features which, together with a glycolipid of low R f value, appear to delineate the members of the Sporomusa-PectinatusSelenomonas phyletic group (Van Golde et al, 1973 ;Johnston & Goldfine, 1982 ;Kamio & Takahashi, 1980 ;Prins et al, 1974 ;Shah et al, 1983 ;Stro$ mpl et al, 1999 ;Verkley et al, 1975 ;Watanabe et al, 1982). While the major polar lipids indicated that C. quercicolum is a member of this evolutionary group, the presence of additional polar lipids enables further differentiation within this group, based on data currently available.…”

“…Fatty acids were analysed as the methyl ester derivatives, as described elsewhere (Stro$ mpl et al, 1999 ;B. J. Tindall, unpublished data).…”

Desulfurispirillum alkaliphilum gen. nov. sp. nov., a novel obligately anaerobic sulfur- and dissimilatory nitrate-reducing bacterium from a full-scale sulfide-removing bioreactor

“…Polar lipids were separated by two-dimensional chromatography and identified on the basis of their R f values in combination with their reaction with specific staining reagents (Tindall 1990a, b). Fatty acid methyl esters were released from 20 mg freeze-dried cells using methodologies, which release only ester linked fatty acids or ester and amide linked fatty acids (Labrenz et al 2005;Strö mpl et al 1999).…”