A reagentless DNA biosensor based on cathodic electrochemiluminescence at a C/CxO1−x electrode

Wu, Aihong; Sun, Jian‐Jun; Zheng, Ruijuan; Yang, Huang‐Hao

doi:10.1016/j.talanta.2010.01.040

Cited by 24 publications

(15 citation statements)

References 33 publications

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“…Indeed, even though they are lower values than previously reported (Fan et al, 2011;Komarova et al, Reisberg et al, 2005;Wu et al, 2010;Zhang et al, 2012) it is noteworthy the fact that this method allows to quantify the DNA in a sample. In order to test the applicability of the quantification procedure, a sample of Target 1 with a known concentration of 0.25 M was inserted into the chamber.…”

Section: Analytical Performance Of the Sensing Systemmentioning

confidence: 58%

Biosensor for label-free DNA quantification based on functionalized LPGs

Gonçalves

Moreira

Pereira

et al. 2016

Biosensors and Bioelectronics

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A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62±2nM and the limit of quantification was 209±7nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.

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Section: Analytical Performance Of the Sensing Systemmentioning

confidence: 58%

Biosensor for label-free DNA quantification based on functionalized LPGs

Gonçalves

Moreira

Pereira

et al. 2016

Biosensors and Bioelectronics

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“…Herein, we report our findings that CuO NPs possess AAO mimetic activity, catalyzing the oxidation of AA to dehydroascorbic acid (DHAA) in the presence of O 2 , and that the good AAO mimetic activity and stability of CuO NPs makes them suitable for various applications. To demonstration the applicability of CuO NPs, a fluorimetric sensor for AA detection—based on AA oxidation catalyzed by CuO NPs and DHAA‐induced formation of fluorescent 3‐(1,2‐dihydroxyethyl)furo[3,4‐ b ]quinoxalin‐1‐one (DFQ) from nonfluorescent OPDA—was established . After optimization of the detection response, this simple, low‐cost, selective, and sensitive fluorimetric method yielded satisfactory results for the quantitative sensing of AA in real samples.…”

Section: Introductionmentioning

confidence: 99%

Ascorbate Oxidase Mimetic Activity of Copper(II) Oxide Nanoparticles

Zhuang

et al. 2019

ChemBioChem

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Although oxidase mimetic nanozymes have been widely investigated, specific biological molecules have rarely been explored as substrates, particularly in the case of ascorbate oxidase (AAO) mimetic nanozymes. Herein, we demonstrate for the first time that copper(II) oxide nanoparticles (CuO NPs) catalyze the oxidation of ascorbic acid (AA) by dissolved O2 (as a green oxidant) to form dehydroascorbic acid (DHAA), thus functioning as a new kind of AAO mimic. Under neutral conditions, the Michaelis–Menten constant of CuO NPs (0.1302 mm) is similar to that of AAO (0.0840 mm). Furthermore, the robustness of CuO NPs is greater than that of AAO, thus making them suitable for applications under various conditions. As a demonstration, a fluorescence AA sensor based on the AAO mimetic activity of CuO NPs was developed. To obtain a fluorescent product, o‐phenylenediamine (OPDA) was used to react with the DHAA produced by the oxidation of AA catalyzed by CuO NPs. The developed sensor was cost‐effective and easy to fabricate and exhibited high selectivity/sensitivity with a wide linear range (1.25×10−6 to 1.125×10−4 m) and a low detection limit (3.2×10−8 m). The results are expected to aid in expanding the applicability of oxidase mimetic nanozymes in a variety of fields such as biology, medicine, and detection science.

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“…Besides this, other researchers found application of quenching effect of Fc for DNA hybridization. This biosensor is based on intrinsic cathodic ECL at thin-oxide-covered glassy carbon (C/C x O 1-x ) electrodes [137] and Fc-MB [138]. Great advantage of the biosensor is attributed to its simplicity and cost-effectiveness with ECL generated from the electrode itself.…”

Section: Dna Detection and Quantificationmentioning

confidence: 99%

Applications of Electrochemiluminescence

Parveen

Aslam

et al. 2013

SpringerBriefs in Molecular Science

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The development of electrochemiluminescence (ECL) applications is a growing field, having the potential advantages of ECL over conventional chemiluminescence. ECL has found various applications in immunoassays, DNA probe assays, and aptasensors by employing ECL-active species as labels on biological molecules. The recent use of ECL to detect many chemically, biochemically, clinically, and environmentally important analytes is reviewed.

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A reagentless DNA biosensor based on cathodic electrochemiluminescence at a C/CxO1−x electrode

Cited by 24 publications

References 33 publications

Biosensor for label-free DNA quantification based on functionalized LPGs

Biosensor for label-free DNA quantification based on functionalized LPGs

Ascorbate Oxidase Mimetic Activity of Copper(II) Oxide Nanoparticles

Applications of Electrochemiluminescence

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