A reagentless triplex DNA junctions-based electrochemical DNA sensor using signal amplification strategy of CHA and tetraferrocene

Wang, Mei; Cui, Hanfeng; Hong, Nian; Shu, Qingxia; Wang, Xinru; Hu, Yuan; Wei, Guobing; Fan, Hao; Zhang, Jing

doi:10.1016/j.snb.2022.131496

Cited by 19 publications

(7 citation statements)

References 41 publications

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“…In addition, ultralow concentrations of circulating DNA in physiological matrices complicates the commercial adaptation of these approaches 107 . Ongoing research aims to overcome these challenges and enable in vivo monitoring through signal amplification and advanced probe design (for example, through the incorporation of multiple redox reporters per probe) 108 .…”

Section: Continuous Real-time Monitoringmentioning

confidence: 99%

Biomolecular sensors for advanced physiological monitoring

et al. 2023

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Body-based biomolecular sensing systems, including wearable, implantable and consumable sensors allow comprehensive health-related monitoring. Glucose sensors have long dominated wearable bioanalysis applications owing to their robust continuous detection of glucose, which has not yet been achieved for other biomarkers. However, access to diverse biological fluids and the development of reagentless sensing approaches may enable the design of body-based sensing systems for various analytes. Importantly, enhancing the selectivity and sensitivity of biomolecular sensors is essential for biomarker detection in complex physiological conditions. In this Review, we discuss approaches for the signal amplification of biomolecular sensors, including techniques to overcome Debye and mass transport limitations, and selectivity improvement, such as the integration of artificial affinity recognition elements. We highlight reagentless sensing approaches that can enable sequential real-time measurements, for example, the implementation of thin-film transistors in wearable devices. In addition to sensor construction, careful consideration of physical, psychological and security concerns related to body-based sensor integration is required to ensure that the transition from the laboratory to the human body is as seamless as possible.

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Section: Continuous Real-time Monitoringmentioning

confidence: 99%

Biomolecular sensors for advanced physiological monitoring

et al. 2023

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“…Technologies that can be integrated in an electrochemical biosensor unit such as catalyzed hairpin assembly (CHA) can function without a specific enzyme with shortened cascade process. 49 2.2.1. Biofluids.…”

Section: Assembling Biosensor Devicesmentioning

confidence: 99%

“…Most representative detection methods are consistent temperature enzyme-free amplification, which is realized with continuous use of target chain with shortened cascade reaction time. Technologies that can be integrated in an electrochemical biosensor unit such as catalyzed hairpin assembly (CHA) can function without a specific enzyme with shortened cascade process …”

Section: Assembling Biosensor Devicesmentioning

confidence: 99%

Advanced Self-Powered Biofuel Cells with Capacitor and Nanogenerator for Biomarker Sensing

Yadav,

Patil,

Dutta

2023

ACS Appl. Bio Mater.

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Self-powered biofuel cells (BFCs) have evolved for highly sensitive detection of biomarkers such as noncodon micro ribonucleic acids (miRNAs) in the presence of interfering substrates. Self-charging supercapacitive BFCs for in vivo and in vitro cellular microenvironments represent the most prevalent sensing mechanism for diagnosis. Therefore, self-powered biosensing (SPB) with a capacitor and contact separation with a triboelectric nanogenerator (TENG) offers electrochemical and colorimetric dual-mode detection via improved electrical signal intensity. In this review, we discuss three major components: stretchable self-powered BFC design, miRNA sensing, and impedance spectroscopy. A specific focus is given to 1) assembling of sensors for biomarkers, 2) electrical output signal intensification, and 3) role of supercapacitors and nanogenerators in SPBs. We outline the key features of stretchable SPBs and the sequence of miRNA sensing by SPBs. We have emphasized the need of a supercapacitor and nanogenerator for SPBs in the context of advanced assembly of the sensing unit. Finally, we outline the role of impedance spectroscopy in the detection and estimation of biomarkers. We highlight key challenges in SPBs for biomarker sensing, which needs improved sensing accuracy, integration strategies of electrochemical biosensing for in vitro and in vivo microenvironments, and the impact of miRNA sensing on cancer diagnostics. This article attempts a specific focus on the accuracy and limitations of sensing unit for miRNA biomarkers and associated tool for boosting electrical signal intensity for a potential big step further.

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“…Among the reported methods for chiral discrimination, electrochemical methods are especially attractive since they can easily convert architectural variation into discernible changes in electrochemical signals such as potential, current, and impedance. [8][9][10][11][12][13][14][15] However, no matter which electrochemical method is used, it is necessary to build chiral sensing platforms with enough discrimination capability. 16 Chiral selectors play a pivotal role in the construction of chiral sensing platforms, which are usually composed of organic ligands and metal ions.…”

Section: Introductionmentioning

confidence: 99%