A real-time RT-PCR assay for molecular identification and quantitation of feline morbillivirus RNA from biological specimens

Luca, Eliana De; Crisi, Paolo Emidio; Domenico, Marco Di; Malatesta, Daniela; Vincifori, Giacomo; Tommaso, Morena Di; Guardo, Giovanni Di; G, Di Francesco; Petrini, Antonio; Savini, Giovanni; Boari, Andrea; Lorusso, Alessio

doi:10.1016/j.jviromet.2018.05.002

Cited by 20 publications

(47 citation statements)

References 18 publications

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“…A follow up was possible only from the 5 cats of colonies which succumbed 8-10 months later after the evidence of FeMV RNA intra vitam. Urine samples were still positive for FeMV RNA, confirming the long lasting shedding of FeMV (Sharp et al, 2016;De Luca et al, 2018). Isolation of FeMV in cell cultures has been confirmed, in this study, to be, in general, difficult and time consuming (Sagaguchi et al, 2014;Woo et al, 2012).…”

Section: Discussionsupporting

confidence: 74%

“…A 15-years old domestic stray cat diagnosed with severe nephropathy was hospitalized and found positive for FeMV RNA in the urine (Lorusso et al, 2015). FeMV RNA was evidenced by quantitative real time PCR from the first day of observation up to cat's death which occurred 110 days later (De Luca et al, 2018). FeMV was also recently identified in Northern Italy (Stranieri et al, 2019).…”

Section: Introductionmentioning

confidence: 91%

“…RNAs were purified according to the manufacturer's recommendations and kept at −80°C until testing. All samples were tested by a real-time RT-PCR for detection of FeMV RNA (qPCR FeMV ) previously established by the study group at IZSAM (De Luca et al, 2018).…”

Section: Molecular Detection Of Femvmentioning

confidence: 99%

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Epidemiology, pathological aspects and genome heterogeneity of feline morbillivirus in Italy

Luca

Crisi

Marcacci

et al. 2020

Veterinary Microbiology

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A B S T R A C TFeline morbillivirus (FeMV) is an emerging morbillivirus first described in cats less than a decade ago. FeMV has been associated with chronic kidney disease of cats characterized by tubulointerstitial nephritis (TIN), although this aspect is still controversial and not demonstrated with certainty. To investigate FeMV prevalence and genomic characteristics, an epidemiological survey was conducted in a total number of 127 household cats originating from two Italian regions, Abruzzi and Emilia-Romagna. A total number of 69 cats originating from three feline colonies were also enrolled for the study. Correlation with TIN was investigated by employing a total number of 35 carcasses. Prevalence of FeMV RNA was higher in urine samples collected from cats of colonies (P = 31.8%, CI 95% 22.1-43.6) compared to household cats (P = 8.66%, CI 95% 4.9-14.9) and in young and middle-aged cats while prevalence of FeMV Abs was higher in old cats. Sequences obtained straight from infected biological samples, either partial or complete, cluster into two clades within FeMV genotype 1, distantly related to FeMV genotype 2. Immunohistochemistry analysis of kidney sections of FeMV RNA positive cats revealed immunoreactivity within epithelial cells of renal tubuli and inflammatory cells. However, statistically significant association between FeMV and renal damages, including TIN, was not demonstrated (p= 0.0695, Fisher exact test). By virus histochemistry performed with FeMV-negative feline tissues and a FeMV isolate, tropism for different cellular types such as inflammatory cells residing in blood vessels of kidney and brain, airway epithelial cells, alveolar macrophages and to a lesser extent, the central nervous system, was demonstrated. Additional studies are warranted in order to establish viral tropism and immune response during the early phases of infection and to disentangle the role of FeMV in co-infection processes.

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 91%

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Epidemiology, pathological aspects and genome heterogeneity of feline morbillivirus in Italy

Luca

Crisi

Marcacci

et al. 2020

Veterinary Microbiology

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“…However, this hypothesis seems unlikely since previous studies reporting slightly higher prevalences were done with conventional PCR. Moreover, the overall prevalence recently obtained with qPCR was only slightly higher compared whit the prevalence registered with conventional PCR (De Luca et al, 2018). Unfortunately, we were not able to perform serological tests on the cats used in this study, therefore no data about the seroprevalence are available.…”

Section: Discussionmentioning

confidence: 69%

“…Long-term shedding of FeMV through the urine has been reported, showing similarity between FeMV in cats and canine distemper virus in dogs (Elia et al, 2015). The analysis of a follow up sample demonstrates that the cat either stopped to shed the virus or intermittently shed the virus, as described in a recent study evaluating the viral RNA shedding within a 110 days' time span; although a previous study demonstrated the persistent shedding of FeMV RNA within a two-years period De Luca et al, 2018). However, the above-mentioned study evaluated the type of shedding in one cat only, thus more information on this issue are needed.…”

Section: Discussionmentioning

confidence: 85%

Feline morbillivirus in Northern Italy: prevalence in urine and kidneys with and without renal disease

Stranieri

Lauzi

Dallari

et al. 2019

Veterinary Microbiology

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A B S T R A C TFeline morbillivirus (FeMV) is an emerging virus that was first described in Hong Kong in 2012. Several reports suggested the epidemiological association of FeMV infection with chronic kidney disease (CKD) in cats. The aim of this study was to investigate the presence and the genetic diversity of FeMV as well as the relationship between FeMV infection and CKD in cats from Northern Italy. Urine (n = 81) and kidney samples (n = 27) from 92 cats admitted to the Veterinary Teaching Hospital of the University of Milan between 2014 and 2017 were investigated for FeMV infection. FeMV RNA was detected in one urine sample (1.23%; 95% CI: 0.03-6.68%) and in two kidneys (7.40%; 95% CI: 0.91-24.28%). FeMV RNA was revealed only in urine or kidneys of cats without evidence of CKD. Phylogenetic analysis showed that the three strains clustered with FeMV strains retrieved from public database, forming a distinct sub-cluster of FeMV. The presence of distinct genotypes of FeMV found in this study is in accordance with previous studies demonstrating that FeMV strains are genetically diverse. A clear relationship between the presence of FeMV infection and CKD in the cats from Northern Italy was not observed, confirming recent reports that do not support the hypothesis that FeMV infection is associated with the development of CKD.

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Molecular characterization of Feline paramyxovirus and Feline morbillivirus in cats from Brazil

et al. 2021

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A real-time RT-PCR assay for molecular identification and quantitation of feline morbillivirus RNA from biological specimens

Cited by 20 publications

References 18 publications

Epidemiology, pathological aspects and genome heterogeneity of feline morbillivirus in Italy

Epidemiology, pathological aspects and genome heterogeneity of feline morbillivirus in Italy

Feline morbillivirus in Northern Italy: prevalence in urine and kidneys with and without renal disease

Molecular characterization of Feline paramyxovirus and Feline morbillivirus in cats from Brazil

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