Recovering energy from biomass and treating organic waste can be done effectively through a biogas digester system. However, monitoring the performance and control of the system to produce biogas is possible through the online process. Therefore, this study developed a novel low-cost gas and temperature profiling system (GTPS) and data acquisition system (DAS) used in biogas digester. The GTPS is designed to monitor and record the amount of methane (CH
4
) and carbon dioxide (CO
2
), the main constituent of biogas, from 0 to 100% and to measure the slurry temperature in the digester using a K-type thermocouple. To test the developed monitoring system, the biogas yield (%) and temperature were monitored, and the result obtained agreed with that reported and obtained in the literature (50–70% and 30−40%) for CH
4
and CO
2
, respectively, followed by the desired mesophilic temperature condition for biogas production (20–35 °C). The study employed an RS-232/RS-485 converter for data communication and a low-cost thermocouple module (isolated analog input) that was installed in the data acquisition system. Interestingly, the application of the converter equipment has never been used in any study on monitoring a biogas digester.