A reappraised meta-analysis of the genetic association between vitamin D receptor BsmI (rs1544410) polymorphism and pulmonary tuberculosis risk

Abstract

BsmI (rs1544410) polymorphism located in intron 8 at the 3′-end of the vitamin D receptor (VDR) gene is known to be involved in the regulation of mRNA stability. Many studies evaluated the possible correlation between VDR BsmI polymorphism and the risk of pulmonary tuberculosis (PTB), and reported conflicting results. In the present study, an updated meta-analysis was performed to evaluate the above-said association. PubMed, Embase, and Google Scholar web-databases were searched for the relevant studies and a meta-analysis was performed by calculating pooled odds ratios (ORs) and 95% confidence intervals (95% CIs) for all the genetic models. A total of 19 studies comprising 3644 controls and 2635 cases were included in the present study. Overall no association of PTB in allelic contrast (b compared with B: P=0.285; OR =0.909, 95% CI =0.762–1.083), homozygous (bb compared with BB: P=0.881; OR =0.975, 95% CI =0.700–1.359), heterozygous (bB compared with BB: P=0.834; OR =1.017, 95% CI =0.872–1.185), dominant (bb compared with BB + Bb: P=0.451; OR =0.954, 95% CI =0.843–1.079) and recessive (bb + Bb compared with BB: P=0.983; OR =1.002, 95% CI =0.868–1.156) genetic models in comparison with wild-type allele and genotype BB were observed. However, variant allele (b compared with B: P=0.001; OR =2.289, 95% CI =1.661–3.154) showed increased risk of PTB in Asians. In conclusion, VDR BsmI polymorphism is not a risk factor for PTB in overall population. However, this polymorphism may be interrelated to an increased risk of PTB amongst Asians.

A meta-analysis of the relationship between vitamin D receptor gene ApaI polymorphisms and polycystic ovary syndrome

“…Prior to reaching a final conclusion, limitations of this meta-analysis should also be acknowledged. [29][30][31] Firstly, we found significant heterogeneity in the overall analysis. Many factors might have contributed to this heterogeneity, e.g., variation in patients' characteristics might have been an important source of heterogeneity.…”

Vitamin D Receptor Gene Polymorphisms Are Associated With Leprosy in Southern Brazil

“…The Bsm I polymorphism has also showed no statistically significant association with leprosy in a couple of studies (37, 38). A recent meta-analysis of this polymorphism and tuberculosis showed that the b-allele was a risk factor for disease development, but this was only observed in the Asian population (39). Regarding Bsm I, Apa I, and Taq I polymorphisms, it is not clear whether they have an individual effect on the expression or function of the VDR.…”

Correlation of vitamin D receptor with bronchial asthma in children

“…The primers, rs7975232 and rs1544410 loci of VDR gene, were selected as candidate loci. The primers were synthesized, of which rs1544410 was in accordance with VDR gene sequence in GenBank, and rs1544410 was completed on the automated DNA synthesizer by Shanghai Biological Engineering Technology Service Co., Ltd. (Shanghai, China), followed by purification with denaturing polyacrylamide gel and detection of single nucleotide polymorphism (SNP) site in products of polymerase chain reaction (PCR) using ABI3730 Sequence Analyzer (USA); the levels of total serum IgE in the two groups were determined by electrochemiluminescence immunoassay, and the related operations were completed strictly according to kits (Huashun Biological Engineering Co., Ltd., Shanghai, China) and the instruments ( 10 , 11 ); iii) detection of lung function. Lung function indexes, including forced expiratory volume in 1 sec (FEV1), the ratio of FEV1 to forced vital capacity (FVC) and peak expiratory flow (PEF), were measured using PowerCube (Germany) pulmonary function instrument before treatment and at 14 days after treatment ( 2 , 12 ).…”