A rearing method forHeliothis armiger confertus, and its use for production of the parasitoidApanteles kazakand two insect viruses

Singh, Pritam; Surrey, Michael; Burgess, Elisabeth P. J.; Carey, G.P.

doi:10.1080/03014223.1982.10423859

Cited by 10 publications

(2 citation statements)

References 11 publications

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“…Our procedures were adapted from those of Carl (1977Carl ( , 1978. Singh et al (1982) andMichael et al (1984) to allow for large-scale production.…”

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confidence: 99%

An Efficient Method of Mass Rearing Two Introduced Parasitoids of Noctuids (Lepidoptera: Noctuidae)

Harrington¹,

Hutchinson²,

Dutch³

et al. 1993

Australian Journal of Entomology

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A method is described for rearing two introduced parasitoids; Cotesia kazak (Telenga) (Hymenoptera: Braconidae) on Helicoverpa spp. (Lepidoptera: Noctuidae) and Hyposoter didymator Thunberg (Hymenoptera: Ichneumonidae) on cluster caterpillar, Spodoptera litura (F.) (Lepidoptera: Noctuidae).It facilitates the production of large numbers of parasitoids for field release.A number of species of noctuids are considered to be major pests of agriculture in Western Australia. In response to this problem several species of hymenopteran parasitoids have been introduced and reared for field release. This paper describes the methods employed in mass rearing two of the species; Cotesia kuzak (Telenga) ( = Apanteles kazak Telenga 1949), using Helicoverpa armigera (Hiibner) and H. punctigera (Wallengren) as hosts, and Hyposoter didymator Thunberg on Spodoptera litura (F.). Our procedures were adapted from those of Carl (1977Carl ( , 1978. Singh et al. (1982) and Michael et al. (1984) to allow for large-scale production. General rearing proceduresHosts and parasitoids were both reared in a quarantine insectary. The host colonies were developed from field-collected adults, while pupae imported from Switzerland (C. kuzak) and Greece (H. didymator) initiated the parasitoid colonies. Larvae of the host species were laboratory reared on an artificial diet similar to that described by Shorey and Hale (1%5), but based on soybean, bran, wheatgerm and yeast.Room temperature was 15°C. except for two periods of the day (0500-0830 and 1500-2000 h) when it was 25 "C to encourage oviposition. Natural light was supplemented by fluorescent lighting to provide 15 h light. Relative humidity was 70 & 15%. Rearing of Cotesia kazakWe presented 100 Helicoverpa larvae (late first and second instar) to five female and five male C. kazak ( > 2 d old) in 2 L plastic bottles with a mesh base (Fig. 1). The bottles were positioned on their sides with the mesh end facing the light and contained two pieces of host diet (5 mm') and two pieces of folded greaseproof paper (as larval retreats). Five thin streaks of honey were scraped onto the mesh window as food for the wasps. The insects remained in the jar for 24 h, after which the parasitoids were removed to perspex holding cages supplied with honey. Transfer of adult C. kazuk was achieved using a vacuum pump.FIG. I-Diagram of set-up used for facilitating oviposition of host larvae by C. kazak.Host larvae were allowed to develop to third instar, at which time they were moved (using a fine paintbrush) to 2 L plastic boxes with mesh inserts in their lids. Each box was supplied with two pieces of host diet and two coloured paper napkins. Parasitoid pupae were conspicuous on the coloured background and could be cut away with scissors, while those on the surfaces of the container were levered off by fingernail without mortality. Pupae were placed in paper cups inside perspex cages where they emerged 12-20 d after oviposition, or up to 180 d later under diapause-inducing conditions (< 12 h light). Unparas...

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“…Our procedures were adapted from those of Carl (1977Carl ( , 1978. Singh et al (1982) andMichael et al (1984) to allow for large-scale production.…”

mentioning

confidence: 99%

An Efficient Method of Mass Rearing Two Introduced Parasitoids of Noctuids (Lepidoptera: Noctuidae)

Harrington¹,

Hutchinson²,

Dutch³

et al. 1993

Australian Journal of Entomology

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“…Heliothis were sourced from a laboratory colony (HortResearch, Auckland) and M. croceipes was sourced from both Gisborne and Hawke's Bay lucerne crops and a laboratory colony (Southern Insect Management Laboratory, Mississippi). Methods used for rearing were based on those described by Singh et al (1982) for heliothis and Lewis and Burton (1970) for M. croceipes. Heliothis larvae were reared individually from the second instar on a lima bean diet (McManus et al 1995).…”

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confidence: 99%

Introduction and establishment of Microplitis croceipes a larval parasitoid of heliothis in North Island pine forests

Herman¹,

Davidson²

2000

NZPP

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Heliothis (Helicoverpa armigera) is a periodic pest of young pine trees (Pinus radiata) In response to a recent outbreak of this pest Microplitis croceipes a parasitoid of heliothis larvae was introduced into forests of the North Island volcanic plateau to augment biological control of heliothis A total of 13600 M croceipes pupae were released at monthly intervals over three seasons (199598) Successful overwintering of M croceipes populations confirmed establishment in the forests Mean monthly parasitism reached 107 in the third season Surveys at points radiating out from the release sites indicated that M croceipes was spreading at approximately 1 km per year

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Influence of the food plants on the degree of parasitism of larvae ofHeliothis armigera byCotesia kazak

et al. 1988

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Influence of the food plants of Heliothis armigera (Hb.) on the degree of parasitism by exotic parasite Cotesia kazak Telenga (Hymenoptera : Braconidae) was studied in cages in the laboratory on 7 food plants such as cotton (Gossypium hirsuture L.), tomato (L ycopersicon esculentum Mill), okra [Abelmoschus esculentus (L.) Moench], Dolichos (Dolichos lablab L.), pigeonpea [Cajanus cajan (L.) Millsp.], Cowpea (Vigna unquiculata (L.) and chickpea (Cicer arietium L.). To

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A rearing method forHeliothis armiger confertus, and its use for production of the parasitoidApanteles kazakand two insect viruses

Cited by 10 publications

References 11 publications

An Efficient Method of Mass Rearing Two Introduced Parasitoids of Noctuids (Lepidoptera: Noctuidae)

An Efficient Method of Mass Rearing Two Introduced Parasitoids of Noctuids (Lepidoptera: Noctuidae)

Introduction and establishment of <i>Microplitis croceipes</i> a larval parasitoid of heliothis in North Island pine forests

Influence of the food plants on the degree of parasitism of larvae ofHeliothis armigera byCotesia kazak

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