This paper attempts to resolve the problem concerning the interval observers design for linear systems with ostensible Metzler system matrices. Because system dynamics matrices are partially different from strictly Metzler structures, a solution is achieved by constructing a composed system matrix representation, which combines pre-compensated interval matrix structures fixed with a prescribed region of D-stability and the reconstructed strictly Metzler matrix structure, related to the original interval system matrix parameter definition. A novel design procedure is presented, which results in a strictly positive observer gain matrix and guarantees that the lower estimates of the positive state variables are non-negative when considering the given system structure and the non-negative system state initial values. The design is computationally simple since it is reduced to the feasibility of the set of linear matrix inequalities.