A recessive gene controlling male sterility sensitive to short daylength/low temperature in wheat (Triticum aestivum L.)

Abstract

Utilization of a two-line breeding system via photoperiod-thermo sensitive male sterility has a great potential for hybrid production in wheat (Triticum aestivum L.). 337S is a novel wheat male sterile line sensitive to both short daylength/low temperature and long daylength/high temperature. Five F(2) populations derived from the crosses between 337S and five common wheat varieties were developed for genetic analysis. All F(1)'s were highly fertile while segregation occurred in the F(2) populations with a ratio of 3 fertile:1 sterile under short daylength/low temperature. It is shown that male sterility in 337S was controlled by a single recessive gene, temporarily designated as wptms3. Bulked segregant analysis (BSA) coupled with simple sequence repeat (SSR) markers was applied to map the sterile gene using one mapping population. The wptms3 gene was mapped to chromosome arm 1BS and flanked by Xgwm413 and Xgwm182 at a genetic distance of 3.2 and 23.5 cM, respectively. The accuracy and efficiency of marker-assisted selection were evaluated and proved essential for identifying homozygous recessive male sterile genotypes of the wptms3 gene in F(2) generation.

Transcriptome analysis reveals new microRNAs-mediated pathway involved in anther development in male sterile wheat

“…Further studies have proven that 337S is a male sterile line that is sensitive to both long day-length/high temperature and short day-length/low temperature condition, but it is fertile at normal planting time. These characteristics indicated that the fertility change of 337S line is due to the thermo-light condition of its cultivated environment, and 337S is a novel bi-pole-photo-thermo-sensitive genic male sterile (BP-PTGMS) line in wheat as described previously [ 19 , 20 ]. In order to explore the mechanism of male sterility in 337S line at short day-length/low temperature condition, we characterized the roles of miRNAs and their targets in BP-PTGMS of wheat, using high-throughput small RNA and degradome sequencing analyses.…”

Development of genic SSR markers from transcriptome sequencing of pear buds

“…By virtue of their reproducibility, multi-allelic nature, co-dominant inheritance, relative abundance, and good genome coverage (Powell et al, 1996), SSR markers have been largely applied to genetic diversity studies (Gupta and Varshney, 2000;Chen et al, 2011;Zhang et al, 2013) and used in a variety of applications related to pears, demonstrating their adaptability for Pyrus (Yamamoto et al, 2002;Bao et al, 2007;Katayama et al, 2007;Yao et al, 2010;Cao et al, 2012). SSR loci consist of randomly repeated DNA regions with motif lengths of one to six base pairs (bp) and are spread throughout the genome (Tóth et al, 2000).…”

Geochemistry, health risk assessment and statistical source identification of dissolved trace elements in surface water of the Chishui River, China

“…Based on the published studies, 62,63 if the correlation coefficient between the element factors is positive, these factors may have a common source, mutual dependence, and identical behaviour during the transport. 64 Specifically, the highly significant positive correlations observed between these elements in river monitoring results may reveal multifaceted environmental dynamics and material behavior. Such positive correlations may reflect several aspects.…”