A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus

Johnson, Michael A.; Pooley, Catherine; Ignjatovic, Jagoda; Tyack, Scott G.

doi:10.1016/s0264-410x(03)00227-5

Cited by 111 publications

(92 citation statements)

References 23 publications

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“…This initiated interest in the development of nonhuman AdVs, including FAdVs, which are an attractive choice both as vaccine vectors for poultry (15,16) and as gene therapy vectors. The optimization of delivery routes and application regimens of AdV vectors are also needed to counteract the limitations of HAdV-based vaccines (21).…”

Section: Discussionmentioning

confidence: 99%

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Oral Inoculation of Chickens with a Candidate Fowl Adenovirus 9 Vector

Deng

Sharif

Nagy

2013

Clin Vaccine Immunol

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Fowl adenoviruses (FAdVs) are a potential alternative to human adenovirus-based vaccine vectors. Our previous studies demonstrated that a 2.4-kb region at the left end of the FAdV-9 genome is nonessential for virus replication and is suitable for the insertion or replacement of transgenes. Our in vivo study showed that the virus FAdV-9⌬4, lacking six open reading frames (ORFs) at the left end of its genome, replicates less efficiently than wild-type FAdV-9 (wtFAdV-9) in chickens that were infected intramuscularly. However, the fecal-oral route is the natural route of FAdV infection, and the oral administration of a vaccine confers some advantages compared to administration through other routes, especially when developing an adenovirus as a vaccine vector. Therefore, we sought to investigate the effects of FAdV-9 in orally inoculated chickens. In the present study, we orally inoculated specific-pathogen-free (SPF) chickens with FAdV-9 and FAdV-9⌬4 and assessed virus shedding, antibody response, and viral genome copy number and cytokine gene expression in tissues. Our data showed that FAdV-9⌬4 replicated less efficiently than did wtFAdV-9, as evidenced by reduced virus shedding in feces, lower viral genome copy number in tissues, and lower antibody response, which are consistent with the results of the intramuscular route of immunization. Furthermore, we found that both wtFAdV-9 and FAdV-9⌬4 upregulated the mRNA expression of alpha interferon (IFN-␣), IFN-␥, and interleukin-12 (IL-12). In addition, there was a trend toward downregulation of IL-10 gene expression caused by both viruses. These findings indicate that one or more of the six deleted ORFs contribute to modulating the host response against virus infection as well as virus replication in vivo.

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Section: Discussionmentioning

confidence: 99%

“…FAdVs are also suitable vectors; for example, FAdV-1-and FAdV-8-based recombinant viruses have induced protective immune responses against infectious bursal disease virus and infectious bronchitis virus, respectively (15,16).…”

mentioning

confidence: 99%

Oral Inoculation of Chickens with a Candidate Fowl Adenovirus 9 Vector

Deng

Sharif

Nagy

2013

Clin Vaccine Immunol

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“…S1 has also been expressed in birds using fowl pox virus [99] and fowl adenovirus vectors [53]. Remarkably, expression of S1 in birds using a nonpathogenic fowl adenovirus vector induced protection in 90% and 100% of chickens in two experiments.…”

Section: Vector Vaccinesmentioning

confidence: 99%

“…This leaves us far short of understanding the molecular basis of cross-protection -or lack of itas cellular immune responses play a role in protection, and these are poorly understood. We do know that the S protein alone is sufficient to induce good protective immunity [12,20,46,51,53,93,96]. There is increasing evidence that only a few amino acid differences amongst S proteins are sufficient to have a detrimental impact on cross-protection.…”

Section: Introductionmentioning

confidence: 99%

Coronavirus avian infectious bronchitis virus

2007

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-Infectious bronchitis virus (IBV), the coronavirus of the chicken (Gallus gallus), is one of the foremost causes of economic loss within the poultry industry, affecting the performance of both meat-type and egg-laying birds. The virus replicates not only in the epithelium of upper and lower respiratory tract tissues, but also in many tissues along the alimentary tract and elsewhere e.g. kidney, oviduct and testes. It can be detected in both respiratory and faecal material. There is increasing evidence that IBV can infect species of bird other than the chicken. Interestingly breeds of chicken vary with respect to the severity of infection with IBV, which may be related to the immune response. Probably the major reason for the high profile of IBV is the existence of a very large number of serotypes. Both live and inactivated IB vaccines are used extensively, the latter requiring priming by the former. Their effectiveness is diminished by poor cross-protection. The nature of the protective immune response to IBV is poorly understood. What is known is that the surface spike protein, indeed the amino-terminal S1 half, is sufficient to induce good protective immunity. There is increasing evidence that only a few amino acid differences amongst S proteins are sufficient to have a detrimental impact on cross-protection. Experimental vector IB vaccines and genetically manipulated IBVs -with heterologous spike protein genes -have produced promising results, including in the context of in ovo vaccination.

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“…In poultry, fowl adenovirus (FAdV) has been used to express a number of complex viral antigens (Sheppard et al, 1998;Johnson et al, 2003;Francois et al, 2004) and eukaryotic proteins including chicken interferon-g (Rauw et al, 2007), interleukin-2 (Cherenova et al, 2004) and thymidine kinase (Shashkova et al, 2005). These reports suggest that FAdV could be used as an effective delivery vector for complex eukaryotic proteins such as antibody fragments, with the added potential for prolonged in vivo release due to the replicative ability of the virus.…”

Section: Introductionmentioning

confidence: 99%

Antibody fragments, expressed by a fowl adenovirus vector, are able to neutralize infectious bursal disease virus

et al. 2010

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Single-chain variable fragments (scFv) contain the heavy and light chain variable domains of immunoglobulin, joined by a short peptide linker. Previously, our laboratory has produced neutralizing scFv to epitopes of infectious bursal disease virus (IBDV). The in vitro delivery and expression of one of these scFv with and without the C H 2-C H 4 Fc domain of chicken IgY attached (scFv-Fc) by a serotype 8 fowl adenovirus (FAdV-8) vector was investigated in the present study. A panel of FAdV-8 vectors was constructed, each containing a different transgene (scFv or scFv-Fc), a different promoter to drive scFv and scFv-Fc transcription (CMVie or the fowl adenovirus major late promoter), and a different sized, right-hand end genomic deletion (52 bp or 2.3 kb). This panel was used to establish what effect these variables had on protein production, viral replication and scFv transcription, as measured by enzyme-linked imunosorbent assay and real-time polymerase chain reaction. Our results showed that, using a FAdV-8 vector containing the optimal CMVie promoter/2.3 kb deletion combination, we successfully expressed a secreted form of both scFv and scFv-Fc that were able to neutralize IBDV both in vitro and in ovo. These studies indicate that the FAdV-8 vector may be a promising candidate to deliver and express therapeutic molecules such as scFv and scFv-Fc in vivo in poultry.

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A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus

Cited by 111 publications

References 23 publications

Oral Inoculation of Chickens with a Candidate Fowl Adenovirus 9 Vector

Oral Inoculation of Chickens with a Candidate Fowl Adenovirus 9 Vector

Coronavirus avian infectious bronchitis virus

Antibody fragments, expressed by a fowl adenovirus vector, are able to neutralize infectious bursal disease virus

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