A recombinant isoform of the Ole e 7 olive pollen allergen assembled by de novo mass spectrometry retains the allergenic ability of the natural allergen

Oeo-Santos, Carmen; Mas, Salvador; Benedé, Sara; López-Lucendo, María; Quiralte, Joaquín; Blanca, Miguel; Mayorga, Cristobalina; Villalba, Mayte; Barderas, Rodrigo

doi:10.1016/j.jprot.2018.06.001

Cited by 11 publications

(21 citation statements)

References 33 publications

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“…Importantly, all of the previously reported allergen families were found (17)(18)(19)57). Only the allergen Ole e 6 was not observed.…”

Section: Discussionmentioning

confidence: 74%

“…Indirect ELISA was performed in 96-well plates (Costar, Corning, New York, US) coated with 0.1 μg recombinant protein per well, according to previously optimized protocols (18,38) For the IgE inhibition experiments with indicated protein extracts, a pool of sera (n=5 for WB; n=6 for ELISA) was pre-incubated with 5 mg/mL for WB; or alternatively 0.25 and 2.5 mg/mL for ELISA of the inhibiting extracts for 2 h at room temperature and shaking. The following steps of the ELISA or WB experiments were performed as described above and the results shown as percentage of inhibition (18,38). For the IgE inhibition experiments using purified proteins, serum was pre-incubated with 10 μg of rOle e 15, nOle e 1 or both.…”

Section: Immunological Characterizationmentioning

confidence: 99%

“…sylvestris proteins sharing the highest sequence identity with Allergome allergens of the indicated allergen families (see also Table S7). ** Ole e 7 sequence has been recently published and is not yet deposited in Uniprot (18). *** Cytosolic malate dehydrogenase from ash (F. excelsior) pollen, a member of the Oleaceae family, was suggested as potential allergen in (39).…”

Section: Olive Pollen Allergenome Delineation Reveals the Presence Ofmentioning

confidence: 99%

“…Several regions of these countries reach extremely high olive pollen counts in the pollination season, becoming olive pollen a major allergenic health problem (16). Until now, thirteen olive pollen allergens, Ole e 1 to Ole e 12 and Ole e 14, have been identified (17)(18)(19). However, some allergens remain unidentified due to technical restrictions and limited genomic and proteomic data.…”

Section: Introductionmentioning

confidence: 99%

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Delineation of the Olive Pollen Proteome and Its Allergenome Unmasks Cyclophilin as a Relevant Cross-Reactive Allergen

et al. 2019

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Olive pollen is a major allergenic source worldwide for its extensive cultivation. We have combined available genomic data with a comprehensive proteomic approach to get the annotated olive tree (Olea europaea L.) pollen proteome and define its complex allergenome. A total of 1,907 proteins were identified by LC-MS/MS using predicted protein sequences from its genome. Most proteins (60%) were predicted to possess catalytic activity and be involved in metabolic processes. In total, 203 proteins belonging to 47 allergen families were found in olive pollen. A peptidyl-prolyl cis-trans isomerase-cyclophilin-produced in Escherichia coli, was found as a new olive pollen allergen (Ole e 15). Most Ole e 15-sensitized patients were children (63%) and showed strong IgE recognition to the allergen. Ole e 15 shared high sequence identity with other plant, animal and fungal cyclophilins and presented high IgE cross-reactivity with pollen, plant food and animal extracts.

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“…Importantly, all of the previously reported allergen families were found (17)(18)(19)57). Only the allergen Ole e 6 was not observed.…”

Section: Discussionmentioning

confidence: 74%

Section: Immunological Characterizationmentioning

confidence: 99%

Section: Olive Pollen Allergenome Delineation Reveals the Presence Ofmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Delineation of the Olive Pollen Proteome and Its Allergenome Unmasks Cyclophilin as a Relevant Cross-Reactive Allergen

et al. 2019

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“…Fragment information of secondary mass spectra was analyzed by de novo sequence method . Each component of A, B, and C was analyzed.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of antioxidant peptides generated from Jiuzao (residue after Baijiu distillation) protein hydrolysates and their effect of enhancing healthy value of Chinese Baijiu

et al. 2019

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BACKGROUND: Jiuzao is the residue after Bajiu distillation which is usually used as forage for livestock. However, it is not fully utilized yet considering the content of protein remained. The present study aimed to isolate antioxidant peptides from Jiuzao protein hydrolysates, then add these peptides into Baijiu product to enhance the healthy value of Baiju. Meanwhile environmental pollution caused by massive Jiuzao can be mitigated indirectly. RESULTS: Four peptides Ala-Tyr-Ile(Leu) (AYI(L)) andAsp-Arg-Glu-Ile(Leu) (DREI(L)) were identified from Jiuzao protein hydrolysates, the extraction contents of AYI + AYL and DREI + DREL were 896.10 and 110.51 mg kg −1 Jiuzao, respectively. On the one hand, antioxidant activities of these peptides were investigated. For in vitro antioxidant assays, AYI, AYL and DREI exhibited strong capacities in oxygen radical absorbance capacity (ORAC) assay. Furthermore, three levels of four peptides were assessed by 2,2'-azobis(2-methylpropanimidamidine) (AAPH)-induced HepG2 cells model. The results showed that these peptides exerted a degree of antioxidant activities in cells. Meanwhile, selected peptides concentrations according to cell assays remined at effective doses after in vitro digestion. On the other hand, the influence of these four peptides on the characteristic aroma compounds in Baijiu was studied. Most characteristic aroma compounds releases were increased with the addition of peptides.CONCLUSION: In the study, antioxidant activities of peptides were evaluated, the feasibility of utilizing Jiuzao protein hydrolysates to obtain beneficial peptides was also proved. Healthy effect of Baijiu or other food can be increased by adding these functional substances. The findings might contribute to food application and Baijiu industries.

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Integration of in vitro allergy test results and ratio analysis for the diagnosis and treatment of allergic patients (INTEGRA)

Pascal

Moreno‐Aguilar

Dávila

et al. 2021

Clinical & Translational All

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The introduction of molecular diagnosis into routine clinical practice has substantially improved the diagnosis and management of allergic patients by allowing clinicians to precisely identify the allergenic molecule responsible for immunoglobulin E (IgE)‐mediated allergies. However, it can be challenging to accurately interpret the results of molecular assays, partly due to the limited evidence base. In this context, a panel of experts with extensive experience in interpreting in vitro measures of total and serum specific IgE reviewed the available scientific evidence. After this review, the panel selected a series of representative case studies to demonstrate how determination of specific and total IgE values and the relationship between them (ratio analysis) can add value to the diagnostic process by more precisely defining the patient’s sensitization profile. Finally, the experts developed a series of recommendations on the clinical application of ratio analysis to optimize and complement the classical approach to allergy diagnosis.

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A recombinant isoform of the Ole e 7 olive pollen allergen assembled by de novo mass spectrometry retains the allergenic ability of the natural allergen

Cited by 11 publications

References 33 publications

Delineation of the Olive Pollen Proteome and Its Allergenome Unmasks Cyclophilin as a Relevant Cross-Reactive Allergen

Delineation of the Olive Pollen Proteome and Its Allergenome Unmasks Cyclophilin as a Relevant Cross-Reactive Allergen

Evaluation of antioxidant peptides generated from Jiuzao (residue after Baijiu distillation) protein hydrolysates and their effect of enhancing healthy value of Chinese Baijiu

Integration of in vitro allergy test results and ratio analysis for the diagnosis and treatment of allergic patients (INTEGRA)

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