A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging

Abstract

The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound YZ-A4 as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor YZ-A4 to selenocysteine was from 0 to 32 μM, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that YZ-A4 could be used for fluorescence imaging of selenocysteine in living cells.

Recent progress in the development of small-molecule fluorescent probes for detection and imaging of selenocysteine and application in thyroid disease diagnosis

“…9). 59 The probe L 11 alone exhibited weak emission at 614 nm due to the NBD group which acts as an ICT fluorescence quencher. However, L 11 showed a ∼20-fold increase in the fluorescence emission intensity at 614 nm upon reaction with SeCys among the other amino acids and biothiols studied.…”

Construction Strategy and Imaging of Highly Selective Selenocysteine Fluorescent Probes

“…该探针的不足在于: 由 于磺酸酯基的高活性, 探针在检测 Sec 时将受到生物硫 醇的轻微干扰. 2020 年, 蒋玉仁课题组 [30] 2015 年, 林伟英课题组 [32] 采用近红外染料 HD-NH2 图 8 近红外染料骨架探针 9 对 Sec 的识别及成像 [32] Figure 8 Identification and imaging of Sec by NIR dye skeleton probe 9 [32] 2021 年, 蒋玉仁课题组 [33] 与单光子光源相比, 使用双光子激光器作为光源可 以避免对生物系统的光毒性问题, 实现更深的穿透深 度. 2021 年, 周礼义课题组 [39] 图 14 双光子探针 16 对 Sec 的识别 [39] Figure 14 Identification of Sec by two-photon probe 16 [39] 17 图 15 碳点探针 17 的合成及对 Sec 的识别 [40] Figure 15 Synthesis of carbon dot probe 17 and recognition of Sec [40] 图 16 基于苯醚与 Sec 硒解反应机理…”