A red fluorescent small-molecule for visualization of higher-order cyclic dimeric guanosine monophosphate (c-di-GMP) structure in live bacterial cells and real-time monitoring of biofilm formation on biotic and abiotic surfaces

Zhang, Yihan; Huang, Xuan-He; Wong, Wing‐Leung; Luo, Jun-Ren; Guo, Xiao-Chun; Liu, Wenjie; Hou, Jinqiang; She, Meng‐Ting; Jiang, Wenhao; Sun, Ning; Lu, Yu‐Jing

doi:10.1016/j.snb.2022.132992

Cited by 2 publications

(1 citation statement)

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“…Biofilm development is the most fascinating process for developing increased resistance to destructive environmental factors (Figaj et al 2023). A biofilm is a group of microorganisms grown on biotic and abiotic surfaces, in which various bacterial cells compactly adhere to each other embedded in extracellular polymeric substance (EPS) produced by the group of cells themselves (Zhang et al 2023). The EPS is made up of polysaccharides, DNA and proteins (Das 2022).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Bacteriophage MAC-1 Potential to Control Pseudomonas aeruginosa Planktonic Cells and Biofilms

Aqeel,

Bukhari,

Ullah

et al. 2024

JSM

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Pseudomonas aeruginosa is a pathogenic bacterium that can be considered a high risk to human health due to its remarkable capacity to resist antibiotics, either intrinsically or following the acquisition of resistance genes. P. aeruginosa has been considered a major threat to human health as all the known remedies seem ineffective. Bacteriophages as a natural killer of bacteria can offer alternative therapy for antibiotics. The study aimed to isolate and characterize a lytic bacteriophage against P. aeruginosa (clinically resistant strain/superbug) and to investigate its lytic potential to control bacterial planktonic cells and their biofilms on stainless steel surfaces. A lytic bacteriophage known as MAC-1 was isolated from wastewater against the selected P. aeruginosa-2750 strain. Its host range was moderate and it only infected six isolates. Phage MAC-1 was characterized through transmission electron microscopy and was classified to the Siphoviridae family. It has good heat and pH stability. Its latent time was 24 min with a burst size of about 410 virions per cell. The phage MAC-1 efficacy was determined against P. aeruginosa planktonic cells and biofilms on metallic surfaces. Isolated phage MAC-1 demonstrated promising activity against bacterial planktonic cells as well as in reducing bacterial biofilm biomass formed in 96-well and on stainless steel plates. However, a phage cocktail may be used to avoid resistance and ensure complete eradication of bacterial biofilms.

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Section: Introductionmentioning

confidence: 99%