2013
DOI: 10.5194/bgd-10-9867-2013
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A reduced fraction of plant N derived from atmospheric N (%Ndfa) and reduced rhizobial nifH gene numbers indicate a lower capacity for nitrogen fixation in nodules of white clover exposed to long-term CO<sub>2</sub> enrichment

Abstract: Using the δ15N natural abundance method, we found that the fraction of nitrogen derived from atmospheric N (%Ndfa) in field grown white clover (Trifolium repens L.) plants was significantly lower (72.0% vs. 89.5%, p = 0.047 in a grassland exposed to elevated CO2 for 13 yr using Free Air Carbon Dioxide Enrichment (FACE). Twelve months later we conducted an experiment to investigate the reasons behind the reduced N fixation. We took cuttings from white clover pla… Show more

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Cited by 8 publications
(11 citation statements)
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“…Quantitative PCR (qPCR) of the nifH gene was carried out with a primer set PolFI/AQER on C1000 TM Thermal Cycler equipped with CFX96 TM Real-Time system (Bio-Rad, United States). Thermal cycler protocols and amplification conditions were referred to Watanabe et al (2013) . Briefly, The 25 μl reaction mixture contained 12.5 μl of SYBR ® Premix Ex Taq TM (TaKaRa), primer set (0.5 μM each), 200 ng BSA μl -1 , 1.0 μl template containing approximately 2–9 ng DNA.…”
Section: Methodsmentioning
confidence: 99%
“…Quantitative PCR (qPCR) of the nifH gene was carried out with a primer set PolFI/AQER on C1000 TM Thermal Cycler equipped with CFX96 TM Real-Time system (Bio-Rad, United States). Thermal cycler protocols and amplification conditions were referred to Watanabe et al (2013) . Briefly, The 25 μl reaction mixture contained 12.5 μl of SYBR ® Premix Ex Taq TM (TaKaRa), primer set (0.5 μM each), 200 ng BSA μl -1 , 1.0 μl template containing approximately 2–9 ng DNA.…”
Section: Methodsmentioning
confidence: 99%
“…Indeed, in fertile conditions, where key nutrients were not limiting growth and N 2 -fixation ( Almeida et al. , 2000 ; Lüscher et al ., 2004 ; Watanabe et al. , 2013 ), legumes benefit more from elevated atmospheric CO 2 concentrations than non-fixing species ( Hebeisen et al.…”
Section: Legumes Offer An Option For Adapting To Atmospheric Changementioning
confidence: 99%
“…Second, enhanced inputs of labile C into the soil via litter and/or root exudation may stimulate decomposition and N mineralization by a larger and/or more active microbial community in eCO 2 (so-called priming; Kuzyakov, 2002;Dijkstra et al, 2008;R€ utting et al, 2010;Phillips et al, 2011). Third, eCO 2 may increase N-fixation and, over time, N availability in ecosystems with N-fixing taxa, although whether this effect persists is unclear (Zak et al, 1993;Hungate et al, 2004;Watanabe et al, 2013). The relative importance of the different processes by which eCO 2 may decrease or increase plant N acquisition likely varies among ecosystems with different soil fertility and plant community composition, potentially explaining the large but poorly understood variability in responses of soil N availability and plant N acquisition to eCO 2 (e.g.…”
Section: Introductionmentioning
confidence: 99%