A reduced number of cortical neurons show increased Caldendrin protein levels in chronic schizophrenia

A comparison of the synaptic proteome in human chronic schizophrenia and rat ketamine psychosis suggest that prohibitin is involved in the synaptic pathology of schizophrenia

“…a-Internexin has been reported to be associated with PSDs 32,44-50 but in previous work we could not establish an upregulation of a-internexin protein levels in synapse-enriched fractions from dorsolateral prefrontal cortex in human chronic schizophrenia. 28 a-Internexin has also been identified in other proteomic screens, for example, in prefrontal cortex of human alcoholics 51 and it was hypothesized that phosphorylation significantly shifts its isoelectric point. Thus, the altered a-internexin protein location on gel in schizophrenics might be due to a differential phosphorylation or other posttranslational modification.…”

“…74 Alternatively it is therefore plausible that the alterations in gene expression or total protein levels are not necessarily reflected in altered PSD protein levels, a finding already reported for the synaptic scaffolding protein SAP97. 28 Total cellular SAP97 levels are clearly reduced in the . Merging of total synapses ((e) as red channel) with prohibitin-positive synapses ((f) as green channel) in (g) confirms that prohibitin is in part localized at synaptic sites but only at a subpopulation.…”

The Diversity of Calcium Sensor Proteins in the Regulation of Neuronal Function

“…Postmortem brains of chronic schizophrenics have lower numbers of caldendrin-immunoreactive neurons, which express the protein at a much higher level. This loss of caldendrin in some neurons and up-regulation in others is likely to profoundly change synapto-dendritic signalling in schizophrenic patients (Bernstein et al 2007). Changes in the distribution of caldendrin have also been observed in kainateinduced epileptic seizures in rats.…”

Alternative Splicing, Expression and Cellular Localization of Calneuron-1 in the Rat and Human Brain

“…Processing of human tissue was done as described previously (Bernstein et al 2003(Bernstein et al , 2007. Detection of Calneuron-1 was performed with a 1:500 dilution of the purified, homemade anti-Calneuron-1 rabbit antibody (1:1,000), described above.…”

“…Calneuron-1 immunoreactivity was detected with a mouse polyclonal antibody (H00083698-A01; Abnova, Taipei, Taiwan) used previously (Mikhaylova et al 2009) employing the nickel-enhanced avidin-biotin technique, as described elsewhere (Bernstein et al 1999(Bernstein et al , 2007. For immunohistochemical staining, six whole brain frontal sections from each case were used; these samples were obtained 10-15 years before staining.…”