A reference collection of Japanese aquatic macroinvertebrates

Wakimura, Kei; Takemon, Yasuhiro; Ishiwata, Shin-ichi; Tanida, Kazumi; Abbas, Eman M.; Inai, Koji; Taira, Akikazu; Tanaka, Aki; Kato, Mikio

doi:10.1016/j.egg.2020.100065

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…To date, sequences of seven Hexatoma species, H. (H.) fuscipennis; H. (E.) gressittiana Alexander, 1943; H. (E.) longicornis; H. (H.) nigra Latreille, 1809; H. (E.) nudivena Alexander, 1933; H. (H.) obscura (Meigen, 1818); and H. (E.) spinosa, are deposited in GenBank and BOLD systems as well as sequences of unidentified Hexatoma specimens. In previous studies, Hexatoma sequences were analyzed as a part of barcoding projects [18][19][20][21][22] or phylogenetic analyses of the order Diptera [23,24] or superfamily Tipuloidea [25]. Our study is the first contribution to the Hexatoma larvae taxonomy using phylogenetic analysis based on mitochondrial COI fragment (DNA barcode) and one of the first attempts to reveal phylogenetic relationships between Hexatoma species using molecular markers.…”

Section: Discussionmentioning

confidence: 99%

Immature Stages of Genus Hexatoma (Diptera, Limoniidae) in the Korean Peninsula

et al. 2023

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The genus Hexatoma Latreille, 1809 is a large group of aquatic crane flies, with almost 600 species worldwide. The largest subgenus is Eriocera Macquart, 1838, which includes all nine species known from the Korean Peninsula. Molecular methods were used to associate Hexatoma larvae with their putative adult species from South Korea. Mitochondrial Cytochrome c Oxidase Subunit I (COI) gene fragment sequences (DNA barcodes) of recently collected adults of H. (E.) gifuensis, H. (E.) ilwola, H. (E.) pernigrina, and H. (E.) pianigra were compared with twelve sequences of Hexatoma larvae. The larvae of H. (E.) pernigrina, H. (E.) pianigra, and H. (E.) gifuensis were associated with their putative adults. The larvae of H. (E.) gifuensis and H. (E.) pianigra and the larvae and pupae of H. (E.) pernigrina are described and illustrated. The larvae of two species not associated with any adult are described, and their COI gene fragment sequences (DNA barcodes) are presented. This paper presents the morphological characteristics suitable for distinguishing larval species. A key for the identification of larvae of the genus Hexatoma on the Korean Peninsula has been compiled. H (E.) sachalinensis is recorded from the Korean Peninsula for the first time. Our study is the first contribution to the Hexatoma larvae taxonomy using phylogenetic analysis based on mitochondrial COI fragment (DNA barcode) and one of the first attempts to reveal phylogenetic relationships between Hexatoma species using molecular markers.

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Section: Discussionmentioning

confidence: 99%

Immature Stages of Genus Hexatoma (Diptera, Limoniidae) in the Korean Peninsula

et al. 2023

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“…The specimens were immersed in 70% ethanol until DNA was isolated. DNA of the three bulk community samples (one from ARD and two from SST) were separately extracted using conventional proteinase K treatment/phenol extraction/ethanol precipitation, as previously described [3] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Metabarcoding data of mitochondrial cytochrome c oxidase subunit 1 gene from bulk community of aquatic organisms collected from Nara Prefecture, Japan

Wakimura¹,

Inai²,

Tanida

et al. 2022

Data in Brief

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Genetic variation and population structure of a widely distributed damselfly (Ischnura senegalensis)

Jiang

Zhang

Bai

et al. 2023

Arch Insect Biochem Physiol

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Ischnura senegalensis Rambur, 1842 is among the most widespread damselfly species in the world. Unlike dragonflies with strong migration abilities, I. senegalensis have limited dispersing abilities. Gene flow among I. senegalensis populations may be greatly influenced by anthropogenic disturbance, fragmented suitable habitats, sea straits, or even global warming. In this study, to investigate the genetic diversity of I. senegalensis populations, we sequenced and collected 498 cytochrome oxidase I sequences across the Old World. Haplotype network analysis showed 51 haplotypes and I. senegalensis could be grouped into four regions (Afrotropical region, Oriental region, main Islands of Japan, and the Ryukyu Islands), each of which contains different dominant haplotypes. Based on molecular variance analysis, we found that populations from the Afrotropical region have quite a low gene flow with the Asian populations (except Yemen). Furthermore, rice cultivation may aid the dispersion of I. senegalensis in the oriental region. Populations from the Ryukyu Islands show the highest genetic diversity, which may be due to the geological separation among islands. Our results prove that I. senegalensis has great genetic diversity among different populations across the world.

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A reference collection of Japanese aquatic macroinvertebrates

Cited by 4 publications

References 14 publications

Immature Stages of Genus Hexatoma (Diptera, Limoniidae) in the Korean Peninsula

Immature Stages of Genus Hexatoma (Diptera, Limoniidae) in the Korean Peninsula

Metabarcoding data of mitochondrial cytochrome c oxidase subunit 1 gene from bulk community of aquatic organisms collected from Nara Prefecture, Japan

Genetic variation and population structure of a widely distributed damselfly (Ischnura senegalensis)

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