A Reference Proteomic Database of Lactobacillus plantarum CMCC-P0002

Abstract

Lactobacillus plantarum is a widespread probiotic bacteria found in many fermented food products. In this study, the whole-cell proteins and secretory proteins of L. plantarum were separated by two-dimensional electrophoresis method. A total of 434 proteins were identified by tandem mass spectrometry, including a plasmid-encoded hypothetical protein pLP9000_05. The information of first 20 highest abundance proteins was listed for the further genetic manipulation of L. plantarum, such as construction of high-level expressions system. Furthermore, the first interaction map of L. plantarum was established by Blue-Native/SDS-PAGE technique. A heterodimeric complex composed of maltose phosphorylase Map3 and Map2, and two homodimeric complexes composed of Map3 and Map2 respectively, were identified at the same time, indicating the important roles of these proteins. These findings provided valuable information for the further proteomic researches of L. plantarum.

Effect of inactivation of ccpA and aerobic growth in Lactobacillus plantarum: A proteomic perspective

“…On the contrary, PDH subunit D was expressed also in the wild type strain, regardless of growth condition and, in the mutant strain, it was expressed at lower level under aeration. It is worth to notice that the present study highlighted the expression of PDH subunits in L. plantarum WCFS1 at proteomic level, as it has been very recently observed also in L. plantarum CMCC-P0002 [43].…”

Comparative proteomic analysis of biofilm and planktonic cells of Lactobacillus plantarum DB200

“…Moonlighting activities may be hypothesized for many of the above proteins, as their abundance is predominantly affected at the extra‐cytoplasmic level. Some of these proteins have also been identified in the exoproteome of other lactobacilli . In accordance with the cytoplasmic level of some glycolytic enzymes (e.g., PKI and PGM), the lowest level of catabolite control protein A (CcpA) was found in the biofilm culture; however, a positive role for the master regulator catabolite control protein A in biofilm development was previously reported .…”

Quantitative Proteogenomics and the Reconstruction of the Metabolic Pathway in Lactobacillus mucosae LM1

“…Elongation factors are necessary for the repair of the damaged structural protein, by incorporating amino acids into the growing or impaired polypeptide chain ( Nilsson and Nissen, 2005 ). On the other hand, EF-Tu, and several cytoplasmic proteins (e.g., DnaK, GroES, GroEL, GAPDH), are considered to be multi-functional (called “moonlighting’ proteins), with additional functions usually acquired after modification by glycosylation or phosphorylation ( Le Maréchal et al ., 2015 ; Zhu et al ., 2011 ). In previous studies, EF-Tu was found to play a major role in bacterial colonization of the porcine gut, through its intrinsic property and high affinity for sulfurylated mucus layers ( Danielsen et al ., 2007 ; Nishiyama et al ., 2013 ).…”