A refined-JinQi-JiangTang tablet ameliorates prediabetes by reducing insulin resistance and improving beta cell function in mice

Gao, Lihui; Liu, Quan; Liu, Shuainan; Chen, Zhiyu; Li, Cai-na; Lei, Lei; Sun, Sujuan; Li, Linyi; Liu, Jinglong; Shen, Zhufang

doi:10.1016/j.jep.2013.11.024

Cited by 34 publications

(34 citation statements)

References 52 publications

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“…OGTT is an important index for the evaluation of beta cell function while ITT indicates the insulin sensitivity [57]. The impairment in glucose tolerance and insulin sensitivity during T2DM is reported to be improved by metformin treatment [40, 58]. In the present study, the co-occurring condition impaired glucose tolerance and insulin sensitivity.…”

Section: Discussionmentioning

confidence: 61%

Diazepam Potentiates the Antidiabetic, Antistress and Anxiolytic Activities of Metformin in Type-2 Diabetes Mellitus with Cooccurring Stress in Experimental Animals

Garabadu

Krishnamurthy

2014

BioMed Research International

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Psychological stress is considered as one of the limiting factors in the management of type-2 diabetes mellitus (T2DM). Therefore, the basic objective of the present study was to evaluate the antidiabetic effect of metformin, diazepam, and their combination in cooccurring T2DM and stress condition (DMS). T2DM was induced in the male rats by administering streptozotocin (45 mg/kg, i.p.) and nicotinamide (110 mg/kg, i.p.) with time lag of 15 min. Rats were subjected to two sessions of cold restraint stress paradigm for one hour on the sixth and seventh day after streptozotocin injection. Administration of metformin (25 mg/kg, p.o.) and diazepam (1 mg/kg, p.o.) in combination from the seventh to thirteenth day after streptozotocin injection showed better improvement in glucose tolerance and insulin sensitivity compared to monotherapy of either drug. In addition, the combination significantly attenuated DMS-induced hyperglycemia, hypertriglyceridaemia, hypercorticosteronemia, anxiety-like behavior, and insulin resistance through modulating insulin signaling pathway in the liver compared to monotherapy. Further, improvement of mitochondrial function, integrity, and oxidative stress in hippocampus, hypothalamus, prefrontal cortex, striatum, amygdala, and nucleus accumbens was observed with the combination. Therefore, metformin in combination with diazepam may be a better therapeutic option in the management of T2DM with cooccurring stress condition.

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Section: Discussionmentioning

confidence: 61%

Diazepam Potentiates the Antidiabetic, Antistress and Anxiolytic Activities of Metformin in Type-2 Diabetes Mellitus with Cooccurring Stress in Experimental Animals

Garabadu

Krishnamurthy

2014

BioMed Research International

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“…We previously reported that JQ-R could improve insulin resistance in HFD-induced obesity mouse model, C57BL/6J mice (HFD-C57mice), and found that JQ-R could increase insulin sensitivity by activating the AMPK signaling pathway in liver in this animal model 7 . We also speculated that JQ-R might improve systemic insulin sensitivity via multiple tissues and pathways in different animal and cell models.…”

Section: Discussionmentioning

confidence: 83%

“…It was shown that alkaloids/saponins/polyhydric alcohols at the weight ratio of 15/10/5 achieved an optimal anti-diabetic effect in hyperglycemic and insulin-resistant mice 7 . This ratio was used in this study.…”

Section: Methodsmentioning

confidence: 99%

“…These components were claimed to possess anti-diabetic and anti-inflammatory activities. Our previous studies clearly showed that oral administration of JQ-R significantly decreased fasting blood glucose levels and improved insulin resistance in pre-diabetic high-fat diet (HFD)-induced obesity in C57 mice 7 . Further analysis revealed that JQ-R improved HFD-induced insulin resistance partially via activating the AMPK signaling pathway in both liver and muscle.…”

Section: Introductionmentioning

confidence: 99%

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Anti-diabetic effects and mechanisms of action of a Chinese herbal medicine preparation JQ-R in vitro and in diabetic KK Ay mice

Liu

Gao

et al. 2017

Acta Pharmaceutica Sinica B

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Refined-JQ (JQ-R) is a mixture of refined extracts from Coptis chinensis (Ranunculaceae), Astragalus membranaceus (Leguminosae) and Lonicera japonica (Caprifoliaceae), the three major herbs of JinQi-JiangTang tablet, a traditional Chinese medicine (TCM) formula. The mechanisms by which JQ-R regulates glucose metabolism and improves insulin sensitivity were studied in type 2 diabetic KKAy mice and insulin-resistant L6 myotubes. To investigate the mechanisms by which JQ-R improves insulin sensitivity, a model of insulin-resistant cells induced with palmitic acid (PA) was established in L6 myotubes. Glucose uptake and expression of factors involved in insulin signaling, stress, and inflammatory pathways were detected by immunoblotting. JQ-R showed beneficial effects on glucose homeostasis and insulin resistance in a euglycemic clamp experiment and decreased fasting insulin levels in diabetic KKAy mice. JQ-R also improved the plasma lipid profiles. JQ-R directly increased the activity of superoxide dismutase (SOD) and decreased malondialdehyde (MDA) as well as inducible nitric oxide synthase (iNOS) levels in insulin-resistant L6 cells, and elevated the insulin-stimulated glucose uptake with upregulated phosphorylation of AKT. The phosphorylation levels of nuclear factor kappa B (NF-κB p65), inhibitor of NF-κB (IκB α), c-Jun N-terminal kinase (JNK1/2) and extracellular-signal-regulated kinases (ERK1/2) were also changed after JQ-R treatment compared with the control group. Together these findings suggest that JQ-R improved glucose and lipid metabolism in diabetic KKAy mice. JQ-R directly enhanced insulin-stimulated glucose uptake in insulin-resistant myotubes with improved insulin signalling and inflammatory response and oxidative stress. JQ-R could be a candidate to achieve improved glucose metabolism and insulin sensitivity in type 2 diabetes mellitus.

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“…Radix Astragali is the main herb in many ready-made Chinese medicines for diabetes mellitus, such as the JinQi-Jiangtang tablet [15, 18], and its effective components are considered to be beneficial for diabetes mellitus [19–21]. Both in vitro and in vivo experiments [22, 23] have shown that SR10, an herbal formulation consisting of the aqueous extracts of Radix Astragali , Radix Codonopsis , and barbary wolfberry fruit ( Fructus Lycii ), appears to reduce the apoptosis of streptozotocin-treated pancreatic β-cells and decrease blood glucose levels in a mouse model of type-2 diabetes mellitus through its antioxidative effects.…”

Section: Introductionmentioning

confidence: 99%

Acupuncture paired with herbal medicine for prediabetes: study protocol for a randomized controlled trial

Liu

Feng

et al. 2017

Trials

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BackgroundType-2 diabetes has become a major disease and is known to seriously impair people’s health worldwide. Prediabetes includes impaired fasting glucose (IFG) and impaired glucose tolerance (IGT) and is the most critical period for preventing type-2 diabetes, as it can be identified and reversed. Studies in the past decade have indicated that acupuncture and Chinese herbal medicine may be beneficial for treating prediabetes. However, a randomized controlled trial (RCT) should be conducted to obtain more clinical evidence on this topic.Methods/designAn RCT will be implemented in this study, using a72-week study period (24 weeks for the intervention and 48 weeks for follow-up). Participants will be recruited from the Fifth Affiliated Hospital of Guangzhou Medical University in China. Eighty participants will be randomized to the treatment group (acupuncture plus herbal medicine and health education) or the control group (health education only), 40 participants in each. People included in this study must have been diagnosed with prediabetes using Western medicine criteria. The endpoint indices include the incidence of diabetes mellitus and the reversion rate. The primary outcome is fasting plasma glucose (FPG) level, 2-h plasma glucose (2-hPG) level after a 75-g oral glucose tolerance test (OGTT), and glycosylated hemoglobin (HbA1c) level. Secondary outcomes include the following: Body Mass Index (BMI); hemorheology, including shear rates of whole-blood viscosity and plasma viscosity. Safety indices include hepatic (ALT, AST) and renal function (BUN, Cr) and records of adverse events, including diarrhoea, colds, pharyngitis, and sleep disorders. Quality control will be implemented, including quality control of the laboratory, researchers, participants, investigational drugs, data and documents, occurrence of bias, supervision, among others, according to uniform standard operating procedures (SOPs) which have been established by the Good Clinical Practice (GCP) office of the Fifth Affiliated Hospital of Guangzhou Medical University.DiscussionThe aim of this study is to evaluate the efficacy and safety of acupuncture paired with herbal medicine for the treatment of patients with prediabetes.Trial registrationChinese clinical trials register ChiCTR-INR-16008891. Registered on 23 July 2016.Electronic supplementary materialThe online version of this article (doi:10.1186/s13063-017-2014-4) contains supplementary material, which is available to authorized users.

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A refined-JinQi-JiangTang tablet ameliorates prediabetes by reducing insulin resistance and improving beta cell function in mice

Cited by 34 publications

References 52 publications

Diazepam Potentiates the Antidiabetic, Antistress and Anxiolytic Activities of Metformin in Type-2 Diabetes Mellitus with Cooccurring Stress in Experimental Animals

Diazepam Potentiates the Antidiabetic, Antistress and Anxiolytic Activities of Metformin in Type-2 Diabetes Mellitus with Cooccurring Stress in Experimental Animals

Anti-diabetic effects and mechanisms of action of a Chinese herbal medicine preparation JQ-R in vitro and in diabetic KK Ay mice

Acupuncture paired with herbal medicine for prediabetes: study protocol for a randomized controlled trial

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