A refinery sludge deposition site: presence of nahH and alkJ genes and crude oil biodegradation ability of bacterial isolates

Abstract

204 bacterial isolates from four Greek refinery sludge deposition sites were investigated for the presence of nahH and alkJ genes encoding key enzymes of both aromatic and aliphatic hydrocarbon degradation pathways by PCR and DNA hybridisation. Members of Pseudomonas, Acinetobacter, Bacillus, Rhodococcus and Arthrobacter play important role in bioremediation processes in sandy/loam soil contaminated with oil and nahH and alkJ genes were present in the 73% of the isolates. Consortia of bacterial isolates that were used for biodegradation of aliphatic and aromatic hydrocarbons in crude oil using liquid cultures exhibited rates from 35% to 48% within 10 days of incubation.

Bioremediation of actual soil samples with high levels of crude oil using a bacterial consortium isolated from two polluted sites: investigation of the survival of the bacteria

“…In accordance with our study, other researchers have reported similar results; for example, Fallgren and Jin utilized a bacterial consortium comprised of Pseudomonas, Achromobacter, Bacillus and Micromonospora genera (Fallgren and Jin, 2008). And, Arvanitis et al claimed that Acinetobacter and Rhodococcus strains can be employed in consortium as their ability in petroleum hydrocarbon degradation (Arvanitis et al, 2008). Interestingly, Pseudomonas aeruginosa strain 1242 was isolated from the polluted site of Kharg Island; this species is a typical strain for the production of rhamnolipid, which can improve the bioavailability in soil, and can consume crude oil as the sole carbon and energy source.…”

Characterization and Potentials of Indigenous Oil-Degrading Bacteria Inhabiting the Rhizosphere of Wild Oat (Avena Fatua L.) in South West of Iran.

“…In the present research, previously isolated rhizosphere-inhabiting bacterial strains in wild oat plants growing in contaminated sites were screened and introduced through a series of oil-enriched cultures as the sole carbon and energy sources based on their oil degrading efficacies and capabilities. The strains belonged to different genera previously reported as oil degrading species (8,9,20,28,29). Studies suggested that hydrocarbon contamination enriches oil degrading bacteria (6,10).…”

Evaluation of bioaugmentation and biostimulation effects on the treatment of refinery oily sludge using 2nfull factorial design

“…Although the degradation of hydrocarbons, crude oil and oily sludge by Pseudomonas sp., Acinetobacter sp., and Bacillus sp. is widely reported, 7,14,[24][25][26][27] no previous studies have reported the role of Microbacter sp. or Stenotrophomonas sp.…”