A regimen for obtaining mature human oocytes from donors for research into human fertilization in vitro

Braude, Peter; Bright, Michael V.; Douglas, Charles P; Milton, P. J. D.; Robinson, R. E.; Williamson, J.; Hutchison, J.H.

doi:10.1016/s0015-0282(16)47954-9

Cited by 32 publications

(12 citation statements)

References 15 publications

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“…Samples were produced by masturbation into sterile containers after 48-72 h abstention from intercourse and were prepared within 4 h of ejaculation. Mature human oocytes were collected by aspiration from donors undergoing laparoscopic sterilization, as described by Braude et al [ 4 ] .…”

Section: Methodsmentioning

confidence: 99%

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Preparation of Human Spermatozoa for In Vitro Fertilization by Isopycnic Centrifugation on Self-Generating Density Gradients

Bolton

Braude

1984

Archives of Andrology

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A simple and rapid method is described for the retrieval of hghly motile, morphologically normal spermatozoa from human semen. This method is a modification of a technique described previously [3] and employs the process of isopycnic centrifugation of semen on self-generating Percoll density gradients. The procedure is carried out under sterile conditions and has no detectable deleterious effects on the fertilizing capacity of spermatozoa. Spermatozoa may be recovered from semen samples of widely differing quality and can be used successfully for in vitro fertilization (IVF). This technique may be useful not only for the preparation of spermatozoa for IVF and possibly for artificial insemination by husband (AIH) but also for the investigation and analysis of the causes of infertility associated with oligozoospermia.

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Section: Methodsmentioning

confidence: 99%

“…The fertilizing capacity of spermatozoa recovered from density gradients has been tested in vitro using donor human eggs [4]. So far this test has been performed on 70 occasions.…”

Section: Fertilizing Capacity Of Spermatozoa Prepared By Isopycnic Cementioning

confidence: 99%

Preparation of Human Spermatozoa for In Vitro Fertilization by Isopycnic Centrifugation on Self-Generating Density Gradients

Bolton

Braude

1984

Archives of Andrology

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“…1 (Templeton et al, 1984;Messinis et al, 1985). Other approaches to achieving a supply of mature human oocytes have been described in the context of different research objectives (Braude et al, 1984). In this study 28% of 65 patients recruited did not provide suitable oocytes, either because of inadequate follicular development or because they had already ovulated or started an endogenous LH surge.…”

Section: Resultsmentioning

confidence: 87%

Oocyte recovery and fertilization rates in women at various times after the administration of hCG

Templeton¹,

Look²,

Angell³

et al. 1986

Reproduction

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“…Alternatively, single oocytes can be donated during abdominal surgery such as tubal ligation. Sterilization patients have previously been asked to donate oocytes after mild stimulation (Braude et al, 1984;Messinis et al, 1986).…”

Section: Alternative Sources Of Oocytesmentioning

confidence: 99%

Oocyte donation for stem cell research

Mertes

Pennings

2006

Human Reproduction

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The future success of stem cell research by means of somatic cell nuclear transfer (SCNT) depends on a sufficient supply of human oocytes. However, oocyte donation presents certain risks for the donor, and concerns for women's welfare are rightly vocalized. At the same time, these risks are comparable with the risks faced by other healthy research subjects. Thus, research donation can withstand ethical scrutiny if it fulfils the same conditions as other research involving healthy human subjects. Specifically, this means that the benefits of the research project need to outweigh the harms, that risks must be minimized, that informed consent has to be guaranteed by averting undue inducement and the recruitment of vulnerable women and that donors can and should be reimbursed for their research participation.

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A regimen for obtaining mature human oocytes from donors for research into human fertilization in vitro

Cited by 32 publications

References 15 publications

Preparation of Human Spermatozoa for In Vitro Fertilization by Isopycnic Centrifugation on Self-Generating Density Gradients

Preparation of Human Spermatozoa for In Vitro Fertilization by Isopycnic Centrifugation on Self-Generating Density Gradients

Oocyte recovery and fertilization rates in women at various times after the administration of hCG

Oocyte donation for stem cell research

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