Biofuels are a viable alternative to fossil fuels, providing potential advantages in mitigating greenhouse gas emissions and improving energy security. Nonetheless, the environmental sustainability of biofuels requires thorough evaluation across their whole life cycle. This report provides a thorough Life Cycle Assessment (LCA) of biofuels, emphasizing greenhouse gas emissions, energy use, and resource depletion. Monte Carlo simulation is used to address variability and uncertainty in critical parameters, yielding a reliable assessment of environmental consequences. Findings demonstrate that biofuels may decrease greenhouse gas emissions by 45-60% relative to fossil fuels; however, considerable heterogeneity is seen based on feedstock type, agricultural methods, and conversion technology.