A Reliable and Quick Radial Thin Layer Chromatographie Method for the Diagnosis of Mono- and Disaccharide Disorders

Abstract

Biou et al.r Radial (hin layer chromatography for the diagnosis of mono-and disaccharide disorders 397 Summary: The authors propose a radial thin layer Chromatographie method for the Separation of mono-and disaccharides, which shows better resolution, and is more sensitive (0.25 g for glucose) and less time-consuming (20 min) than the linear technique. Moreover, the procedures used for the detection of sugars and their dansyl-derivatives (staining with naphthoresorcinol and fluorescence of the dansyl-hydrazones) make the identification more specific and reliable. This simple method is quite suitable for the analysis of biological samples (urine, faeces) and the diagnosis of metabolic disorders in children. Eine zuverlässige und schnelle radiale dünnschichtchromatographische Methode zur Diagnose von Mono-und Disaccharid-Stoffwechselstörungen

Carbohydrates

“…These layers are suitable for most classes of sugars and sugar derivatives, the major exception being intact complex polysaccharides. Numerous solvent systems provide relatively good separation of monosaccharides, disaccharides, and lower oligosaccharides (Tables 2 and 3); malto-oligosaccharides (14, 16,43,44); amino sugars (27,40) (Table 4); aminoglycoside antibiotics (44,46); sugar alcohols (48,49) (Table 4); uronic acids (50,51); derivatives (52,53) (Table 4); and cyclodextrins (54). Another advantage of TLC and HPTLC silica gel layers is their chemical stability against almost all solvents, strong acids, and other corrosive reagents used in postchromatographic derivatization procedures.…”