A repeated β-turn structure in Poly(Ala-Gly) as a model for silk I of Bombyx mori silk fibroin studied with two-dimensional spin-diffusion NMR under off magic angle spinning and rotational echo double resonance11Edited by M. F. Summers

Asakura, Tetsuo; Ashida, Jun; Yamane, Tsutomu; Kameda, Tsunenori; Nakazawa, Yasumoto; Ohgo, Kosuke; Komatsu, Kohei

doi:10.1006/jmbi.2000.4394

Cited by 228 publications

(432 citation statements)

References 36 publications

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“…In contrast, three different molecular structures have been identified in Silk II, two antiparallel p-sheet conformations, as major components, and a distorted poly-pll-turn structure [64,65].…”

Section: Study By Cp-mas "C Nmrmentioning

confidence: 98%

“…Analyses by CP-MAS 13 C NMR have also been conducted on B. morí silk fibroin, as well as related model peptides [46,[64][65][66][67][68][69], in order to characterize the secondary structure of SF in the worm gland before spinning (Silk I) and in the spun solid fiber (Silk II). We used degummed SF fibers (D) and lyophilized regenerated SF powder (L) as a reference for Silk II and Silk I structures, respectively (Fig.…”

Section: Study By Cp-mas "C Nmrmentioning

confidence: 99%

“…Silk I structure was initially proposed to be a 3 1 -helixlike conformation [70], but it was later identified as a repeated p-turn type II that is capable of forming intramolecular hydrogen bonds [64,68]. In contrast, three different molecular structures have been identified in Silk II, two antiparallel p-sheet conformations, as major components, and a distorted poly-pll-turn structure [64,65].…”

Section: Study By Cp-mas "C Nmrmentioning

confidence: 99%

“…6), the resonance peaks of the main constituent amino acids of silk fibroin (i.e. Gly, Ala, Ser, Tyr) can be readily identified and their chemical shifts correlated with the molecular conformation of the polypeptide [64,65]. As expected for a p-sheet conformation [59,71,72], Ala Cp peak appears at higher frequency in degummed SF (D) than in lyophilized SF powder (L) (20.7 ppm vs 17.3 ppm, resp.).…”

Section: Study By Cp-mas "C Nmrmentioning

confidence: 99%

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Insights into the production and characterization of electrospun fibers from regenerated silk fibroin

Solanas

Herrero

Dasari

et al. 2014

European Polymer Journal

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Regenerated silk fibroin solutions from Bombyx mori were tested for electrospinning. Simple and reproducible tensile tests were performed on threads of aligned fibers to obtain information about their mechanical performance at the fiber level. The binary solvent formic acid/chloroform (10:1, v/v) rendered unbeaded thinner fibers with increased extensibility before failure when compared with pure formic acid. A remarkable improvement in strength was induced by immersing length-restricted fibers into ethanol for 5 min. Conformational changes of the protein chains were studied by solid-state NMR.

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Section: Study By Cp-mas "C Nmrmentioning

confidence: 98%

Section: Study By Cp-mas "C Nmrmentioning

confidence: 99%

Section: Study By Cp-mas "C Nmrmentioning

confidence: 99%

Section: Study By Cp-mas "C Nmrmentioning

confidence: 99%

See 2 more Smart Citations

Insights into the production and characterization of electrospun fibers from regenerated silk fibroin

Solanas

Herrero

Dasari

et al. 2014

European Polymer Journal

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“…18 The SF has two distinct structures in the solid state: silk I before spinning and silk II after spinning. 19,20 The PU is the solid sample of the dispersion in aqueous solution. In the spectra of the composite materials (Figures 1b-d), all peaks are in the same positions as those of SF and PU (Figures 1a and c).…”

Section: Resultsmentioning

confidence: 99%

Structural characterization of silk-polyurethane composite material for biomaterials using solid-state NMR

Nakazawa

Asano

Nakazawa

et al. 2012

Polym J

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In this paper, we performed solid-state nuclear magnetic resonance (NMR) measurements, including T 1 H and T 1q , of silk fibroin (SF)/polyurethane (PU) composites to examine their possible use as a material for artificial vascular grafts. In the development of new artificial vascular grafts made from SF/PU, it is important to examine the miscibility of the composites and their molecular dynamics, because these properties are intimately involved in the resulting physical properties of the resulting vascular graft. The T 1 H measurements showed that the domain size of the SF/PU ¼ 1:1 composite is smaller than the domain size of the 1:10 and 1:2 composites, indicating that the molecular miscibility between SF and PU are partially in close proximity, particularly in the SF/PU ¼ 1:1 composite. Additionally, we observed that the molecular motion of the soft segment of PU in the SF/PU composites becomes slow, suggesting that the soft segment of PU interacts with SF to some extent. These analyses provided basic structural information for the development of silk-based artificial vascular grafts using PU.

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Silk

Asakura

Kametani

Suzuki

2018

Encyclopedia of Polymer Science and Technology

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Silks are naturally spun fibrous proteins most often associated with silkworms and spiders. For more than 5000 years, silk fiber from Bombyx mori silkworm has been used by mankind as an excellent textile fiber “queen of textiles.” Silk fibers possess excellent mechanical properties due to a combination of strength and extensibility, biocompatibility, and biodegradability. Thus, silks are of high interest for researchers in many fields. Here, we review the current understandings of the production, structures, processing, and properties of silkworms and spider silks. In vivo and in vitro findings in the field of silk research are the basis for the design of new proteins and processing strategies, which will enable applications of these fascinating protein‐based materials in the fields of biomedicine and materials engineering.

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A repeated β-turn structure in Poly(Ala-Gly) as a model for silk I of Bombyx mori silk fibroin studied with two-dimensional spin-diffusion NMR under off magic angle spinning and rotational echo double resonance11Edited by M. F. Summers

Cited by 228 publications

References 36 publications

Insights into the production and characterization of electrospun fibers from regenerated silk fibroin

Insights into the production and characterization of electrospun fibers from regenerated silk fibroin

Structural characterization of silk-polyurethane composite material for biomaterials using solid-state NMR

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