A repetitive Dictyostelium gene family that is induced during differentiation and by heat shock

Zuker, Charles S.; Cappello, Joe; Chisholm, Rex L.; Lodish, Harvey F.

doi:10.1016/0092-8674(83)90557-3

Cited by 45 publications

(50 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transcription of DIRS-1 is regulated during development of D. discoideum (7,22). Figure 1 shows that poly(A)+ DIRS-1-related RNAs were present at very low levels in vegetative cells, below the limit of detection of the RNA filter hybridization experiment (but see lane 2 of the primer extension experiments in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…DIRS-1 RNAs are present at very low levels in growing cells, begin to accumulate within the first hour after initiation of development, and reach their maximal level by 15 h. Transcription of DIRS-1-related RNAs is induced in vegetative cells subjected to stresses such as heat shock and high cell density (22).…”

mentioning

confidence: 99%

“…Examination of the DNA sequence of the DIRS-1 terminal repeats has led to the identification of a putative heat shock promoter sequence (22,23) homologous to the Drosophila consensus heat shock promoter (13,14). Stress-induced transcription of DIRS-1-related RNAs is presumed to be directed by these sequences.…”

mentioning

confidence: 99%

“…Dictyostelium discoideum AX-3 was grown axenically and plated for development on filters as described previously (1). Growing cells were harvested when the concentration reached 2 x 106 cells per ml.The DIRS-1 genomic clones used here (pB41.6 and pB41.3) are subclones of the A genomic clone SB41 and have been described in detail elsewhere (7,22,24 …”

mentioning

confidence: 99%

“…The DIRS-1 genomic clones used here (pB41.6 and pB41.3) are subclones of the A genomic clone SB41 and have been described in detail elsewhere (7,22,24). pB41.3 contains the entire 4.1 kb of DIRS-1 unique internal sequence flanked by EcoRI sites.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Transcription of Dictyostelium discoideum transposable element DIRS-1.

Cohen¹,

Cappello²,

Lodish³

1984

Mol. Cell. Biol.

View full text Add to dashboard Cite

DIRS-1 is a Dictyostelium discoideum transposable element that contains heat shock promoter sequences in the inverted terminal repeats. We showed that transcription of a 4.5-kilobase polyadenylated RNA initiates at a discrete site within the left-terminal repeat of DIRS-1, downstream from heat shock promoter and TATA box sequences. This RNA represents a full-length transcript of DIRS-1. We describe a cDNA clone that contains the 4.1 kilobases of internal sequence of DIRS-1, a cDNA clone that spans the junction between the internal sequences and the right-terminal repeat, and a cDNA clone that appears to have been transcribed from a rearranged genomic copy of DIRS-1. A second DIRS-1 RNA, named El, is transcribed on the opposite strand of DIRS-1 from the 4.5-kilobase RNA and is under control of the heat shock promoter in the right-terminal repeat. El transcription initiates at multiple positions both within and downstream from the right-terminal repeat. The same transcriptional initiation sites are used during normal development and during heat shock, suggesting that in all cases DIRS-1 transcription is regulated by the heat shock promoters contained within the two terminal repeats.Dictyostelium intermediate repeat sequence 1 (DIRS-1) is a moderately repetitive and apparently transposable element with several unusual features (4,7,15). DIRS-1 consists of 4.1 kilobases (kb) of unique internal sequence flanked by inverted terminal repeats of unequal length, 332 and 360 base pairs (bp) (6, 23). There are about 40 copies of the intact DIRS-1 element interspersed throughout the Dictyostelium genome, as well as an additional 200 copies of related sequences (7). Different Dictyostelium strains have different sequences flanking some of their DIRS-1 elements, indicating that the element had transposed after these strains were separated (7, 15).Transcription of DIRS-1-related sequences results in the production of a heterogeneous population of polyadenylated cytoplasmic RNAs that are differentially expressed during development (7, 24; C. Zuker, Ph.D. thesis, Massachusetts Institute of Technology, Cambridge). DIRS-1 RNAs are present at very low levels in growing cells, begin to accumulate within the first hour after initiation of development, and reach their maximal level by 15 h. Transcription of DIRS-1-related RNAs is induced in vegetative cells subjected to stresses such as heat shock and high cell density (22).Examination of the DNA sequence of the DIRS-1 terminal repeats has led to the identification of a putative heat shock promoter sequence (22, 23) homologous to the Drosophila consensus heat shock promoter (13,14). Stress-induced transcription of DIRS-1-related RNAs is presumed to be directed by these sequences. Cappello et al. (5) showed that a cloned partial copy of DIRS-1 (pB41.6) directs transcription of a heat-shock-inducible RNA in yeasts and that heatinducible transcription of this RNA is dependent on the presence of the DIRS-1 terminal repeat. The isolated terminal repeat also directs heat-inducible transcrip...

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Transcription of Dictyostelium discoideum transposable element DIRS-1.

Cohen¹,

Cappello²,

Lodish³

1984

Mol. Cell. Biol.

View full text Add to dashboard Cite

show abstract

Caffeine and heat shock induce adenylate cyclase in Dictyostelium discoideum

Hagmann¹

1986

The EMBO Journal

View full text Add to dashboard Cite

When vegetative cells of Dictyostelium discoideum were incubated in nutrient medium containing 5 mM caffeine, adenylate cyclase was induced and exceeded control values 30‐fold after 6 h of incubation. The effect was dose‐dependent and blocked by cycloheximide. Contact site A, a developmentally regulated membrane glycoprotein characteristic of aggregation‐competent cells, was not induced by the treatment. The expression of a developmentally regulated gene coding for an unknown protein and hybridizing with the cDNA clone P26E8 was also stimulated, indicating that the regulation of expression occurs at the transcript level. Cells pretreated with caffeine in growth medium, washed and developed in starvation buffer showed an acceleration of development by 2 h as judged by stream formation and the appearance of contact sites A. The same effects were observed when cells were incubated under heat shock conditions (30°C). The results indicate that caffeine stimulates the expression of devevelopmentally regulated early genes, and that their products, together with exogeneous factor(s), initiate subsequent steps of development.

show abstract

Induction of stress proteins in chicken embryo cells by low‐level zinc contamination in amino acid‐free media

Whelan

Hightower

1985

Journal Cellular Physiology

View full text Add to dashboard Cite

It has been reported that chicken embryo cells deprived of exogenous amino acids for 4 hours synthesize stress (heat-shock) proteins. Herein, we show that amino acid deprivation is not sufficient to cause induction of stress proteins. Zinc contaminating a component of commercial cell culture medium used to prepare amino acid-free medium was an inducer in our cultures. In the absence of exogenous amino acids, the concentration of zinc ions needed for half-maximal induction of stress proteins was an order of magnitude lower than the dose required for cells in complete medium. Histidine and cystine, which have high affinities for zinc ions, were the amino acids most effective in blocking the induction of stress proteins by zinc. Problems posed by heavy metal ions in culture media and biologic fluids for searches for in vivo inducers of the cellular stress (heat shock) response are discussed.

show abstract

A repetitive Dictyostelium gene family that is induced during differentiation and by heat shock

Cited by 45 publications

References 28 publications

Transcription of Dictyostelium discoideum transposable element DIRS-1.

Transcription of Dictyostelium discoideum transposable element DIRS-1.

Caffeine and heat shock induce adenylate cyclase in Dictyostelium discoideum

Induction of stress proteins in chicken embryo cells by low‐level zinc contamination in amino acid‐free media

Contact Info

Product

Resources

About