A reproducible <i>in vitro</i> pollen germination medium for recalcitrant cereal rye pollen (<i>Secale cereale</i> L): Effect of tryptone

Jayaprakash, P.; Sheeba, D.; Vikas, V. K.; Sivasamy, M.; Kumar, J. Kishore; Annapoorani, S.; Krishnan, Navaneetha

doi:10.5958/0975-6906.2017.00004.9

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Polyethylene Glycol (Peg)1

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“…This immunosuppressor has been used to overcome self-incompatibility and incongruity in Phaseolus [25] and Vigna [26,27]. It has been used as a component of PGM of pigeonpea for the first time [1] and subsequently in the medium of wheat and rye [2,10].…”

Section: Polyethylene Glycol (Peg)mentioning

confidence: 99%

Pollen Germination in vitro

Jayaprakash¹

2018

Pollination in Plants

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Pollen germination in vitro is a reliable method to test the pollen viability. It also addresses many basic questions in sexual reproduction and particularly useful in wide hybridization. Many pollen germination medium ranging from simple sugars to complex one having vitamins, growth regulators, etc. in addition to various minerals have been standardized to germinate pollen artificially. The different media, successful pollen germination methods, procedures from pollen germination studies with wheat, rye, brinjal, pigeonpea and its wild relatives are discussed.

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