A rescued NADC30-like virus by reverse genetic manipulation exhibits moderate virulence and a promising application perspective

Cao, Zhengda; Chen, Jinxia; Li, Liwei; Liu, Jiachen; Wu, Tong; Zhou, Yue; Tong, Guangzhi; Wang, Guihua; Gao, Fei

doi:10.1016/j.virusres.2022.198801

Cited by 3 publications

(3 citation statements)

References 56 publications

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“…Daily rectal temperature and clinical signs were recorded for each group. The clinical signs were scored from food intake, mental, skin, respiratory status, cough condition, runny nose, neurological symptoms, and stool condition, with each index scored from 0 to 5 points; the more obvious the disease, the higher the score [ 23 ]. Serum samples were collected, and the blood viral load and antibody levels were analyzed.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Pathogenicity of Two Novel PRRSVs Recombined by NADC30-like and NADC34-like Strains in China

Peng

et al. 2022

Viruses

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Porcine reproductive and respiratory syndrome viruses (PRRSVs) pose a serious threat to the swine industry in China, which has caused great difficulties for porcine reproductive and respiratory syndrome (PRRS) immune prevention and control, due to its easily mutable and recombinant nature. In this study, two novel PRRSV strains, which were named GD-H1 and GD-F1, were isolated and fully sequenced from pig farms in Guangdong province, China. The phylogenetic analysis and recombination analysis revealed that the GD-H1 and GD-F1 were generated by the recombination of NADC30-like and NADC34-like strains which were different from the previously prevalent strain. Further pathogenic studies on piglets and sows found that the recombinant strains could cause piglets high fever, loss of appetite and lung lesions, but no piglets died. However, the recombinant strains could cause acute death and abortion in pregnant sow infection models together with average survival rates of 62.5% and 37.5% abortion rates, respectively. These findings indicated that the recombinant strains were extremely pathogenic to sows. Therefore, we report two clinical novel recombinant strains of PRRSV that are different from the traditional epidemic strains in China, which may provide early warning and support for PRRS immune prevention and control.

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Section: Methodsmentioning

confidence: 99%

Characterization and Pathogenicity of Two Novel PRRSVs Recombined by NADC30-like and NADC34-like Strains in China

Peng

et al. 2022

Viruses

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“…Currently, this system has been widely used in viral genome replication and transcription, virulence, target cell infection, pathogenesis, and immune response, opening up new avenues for molecular research on PRRSV. Several studies have reported that a number of reverse genetic platforms of PRRSV-2 strains have been successfully constructed, including VR-2332, HuN4-F112, and JXwn06 (Zhengda et al, 2022).…”

Section: Open Access Edited Bymentioning

confidence: 99%

“…Reverse genetic systems have become an important tool in the field of picornavirus research, particularly supporting the investigation of NADC34-like PRRSV. This advanced technology provides a strong technical basis for examining diverse facets such as viral replication, pathogenic mechanisms, virulence genes, molecular details of viral-host interactions, in vivo functionality of viral proteins, the creation of live viral vectors, and the development of recombinant genetically engineered vaccines (Zhengda et al, 2022).…”

Section: Characterization Of the Recombinant Virusmentioning

confidence: 99%

Development and biological characterization of an infectious cDNA clone of NADC34-like PRRSV

Lin,

Zhou,

Xiao

et al. 2024

Front. Microbiol.

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IntroductionPorcine Reproductive and Respiratory Syndrome virus (PRRSV) causes high abortion rates in gestating sows and stillbirths, as well as high piglet mortality, seriously jeopardizing the pig industry in China and worldwide.MethodsIn this study, an infectious clone containing the full-length genome of NADC34-like PRRSV was constructed for the first time using reverse genetic techniques. The gene was amplified segmentally onto a plasmid, transfected into BHK-21 cells, and the transfected supernatant was harvested and transfected into PAM cells, which showed classical cytopathic effects (CPE).ResultsThe virus rJS-KS/2021 was successfully rescued which could be demonstrated by Western Blot and indirect immunofluorescence assays. Its growth curve was similar to the original strain. Replace the 5’UTR and 3’UTR of rJS-KS/2021 with 5’UTR and 3’UTR of HP-PRRSV (strain SH1) also failed to propagate on MARC-145.DiscussionIn this study, an infectious clone of NADC34-like was constructed by reverse genetics, replacing the UTR and changing the cellular tropism of the virus. These findings provide a solid foundation for studying the recombination of different PRRSVs and the adaption of PRRSVs on MARC-145 in the future.

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PSMB1 Inhibits the Replication of Porcine Reproductive and Respiratory Syndrome Virus by Recruiting NBR1 To Degrade Nonstructural Protein 12 by Autophagy

Bai

Zhou

et al. 2023

J Virol

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A rescued NADC30-like virus by reverse genetic manipulation exhibits moderate virulence and a promising application perspective

Cited by 3 publications

References 56 publications

Characterization and Pathogenicity of Two Novel PRRSVs Recombined by NADC30-like and NADC34-like Strains in China

Characterization and Pathogenicity of Two Novel PRRSVs Recombined by NADC30-like and NADC34-like Strains in China

Development and biological characterization of an infectious cDNA clone of NADC34-like PRRSV

PSMB1 Inhibits the Replication of Porcine Reproductive and Respiratory Syndrome Virus by Recruiting NBR1 To Degrade Nonstructural Protein 12 by Autophagy

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