To enhance speed and agility in interpreting physiological quality tests of seeds, The use of algorithms has emerged. This study aimed to identify suitable machine learning models to assist in the precise management of seed lot quality. Soybean lots from two companies were assessed using the Supplied Test Set, Cross-Validation (with 8, 10, and 12 folds), and Percentage Split (with 66% and 70%) methods. Variables analyzed through Tetrazolium tests included vigor, viability, mechanical damage, moisture damage, bed bug damage, and water content. Method performance was determined by Kappa, Precision, and ROC Area metrics. Classification Via Regression and J48 algorithms were employed. The technique utilizing 66% of data for training achieved 93.55% accuracy, with Precision and ROC Area reaching 94.50% for the J48 algorithm. Applying the crossvalidation method with 10 folds resulted in 90.22% of correctly classified instances, with a ROC Area outcome like the previous method. Tetrazolium Vigor was the primary attribute used. However, these results are specific to this study's database, and careful planning is necessary to select the most effective application methods.